Proteomic Analysis of Human Tendon and Ligament : Solubilization and Analysis of Insoluble Extracellular Matrix in Connective Tissues

• Material Type: 博士論文
• ISSN: 1535-3893
• Title: Proteomic Analysis of Human Tendon and Ligament : Solubilization and Analysis of Insoluble Extracellular Matrix in Connective Tissues
• Volume: 15 12
• Author/Editor: Sato, Nori; Taniguchi, Takako; Goda, Yuichiro; Kosaka, Hirofumi; Higashino, Kosaku; Sakai, Toshinori; Katoh, Shinsuke; Yasui, Natsuo; Sairyo, Koichi; Taniguchi, Hisaaki
• Author Heading: Sato, Nori; Taniguchi, Takako; Goda, Yuichiro; Kosaka, Hirofumi; Higashino, Kosaku; Sakai, Toshinori; Katoh, Shinsuke; Yasui, Natsuo; Sairyo, Koichi; Taniguchi, Hisaaki
• Publication, Distribution, etc.: ACS Publications
• Publication Date: 2016-10-17
• Publication Date (W3CDTF): 2016-10-17
• Alternative Title: ヒト腱・靭帯のプロテオーム解析 : 結合組織における不溶性細胞外マトリックスの可溶化と解析
• Periodical title: Journal of Proteome Research
• Degree grantor/type: 徳島大学
• Date Granted: 2017-02-23
• Date Granted (W3CDTF): 2017-02-23
• Dissertation Number: 甲第3009号
• Degree Type: 博士（医学）
• Conferring No. (Dissertation): 甲第3009号
• Text Language Code: eng
• Subject Heading: 不溶性細胞外マトリックス; コラーゲン; エラスチン; プロテオーム解析; yellow ligament; Achilles tendon; elastin; collagen; proteome
• Target Audience: 一般
• Note (General): Connective tissues such as tendon, ligament and cartilage are mostly composed of extracellular matrix (ECM). These tissues are insoluble, mainly due to the highly cross-linked ECM proteins such as collagens. Difficulties obtaining suitable samples for mass spectrometric analysis render the application of modern proteomic technologies difficult. Complete solubilization of them would not only elucidate protein composition of normal tissues but also reveal pathophysiology of pathological tissues. Here we report complete solubilization of human Achilles tendon and yellow ligament, which is achieved by chemical digestion combined with successive protease treatment including elastase. The digestion mixture was subjected to liquid chromatography-mass spectrometry. The low specificity of elastase was overcome by accurate mass analysis achieved using FT-ICR-MS. In addition to the detailed proteome of both tissues, we also quantitatively determine the major protein composition of samples, by measuring peak area of some characteristic peptides detected in tissue samples and in purified proteins. As a result, differences between human Achilles tendon and yellow ligament were elucidated at molecular level.
• DOI: info:doi/10.1021/acs.jproteome.6b00806; https://doi.org/info:doi/10.1021/acs.jproteome.6b00806
• Persistent ID (NDL): info:ndljp/pid/11115931; https://dl.ndl.go.jp/pid/11115931
• Collection: 障害者向け資料
• Collection (Materials For Handicapped People:1): テキストデータ
• Collection (particular): 国立国会図書館デジタルコレクション > デジタル化資料 > 博士論文; https://dl.ndl.go.jp/collections/A00014
• Acquisition Basis: 博士論文（自動収集）
• Date Accepted (W3CDTF): 2018-07-03T21:49:10+09:00
• Format (IMT): application/pdf
• Access Restrictions: 国立国会図書館内限定公開
• Service for the Digitized Contents Transmission Service: 図書館・個人送信対象外
• Availability of remote photoduplication service: 可
• Periodical Title (URI): http://repo.lib.tokushima-u.ac.jp/110137
• Data Provider (Database): 国立国会図書館 : 国立国会図書館デジタルコレクション; https://dl.ndl.go.jp