Spontaneous epileptic seizures in transgenic rats harboring a human ADNFLE missense mutation in the β2-subunit of the nicotinic acetylcholine receptor

• Material Type: 博士論文
• Title: Spontaneous epileptic seizures in transgenic rats harboring a human ADNFLE missense mutation in the β2-subunit of the nicotinic acetylcholine receptor
• Author/Editor: Shiba, Yuko
• Author Heading: Shiba, Yuko
• Publication Date: 2015-09-30
• Publication Date (W3CDTF): 2015-09-30
• Alternative Title: ヒト常染色体優性夜間前頭葉てんかん由来ニコチン性アセチルコリン受容体 β2 サブユニット変異ラットの自発性発作; ヒト ジョウセンショクタイ ユウセイ ヤカン ゼントウヨウ テンカン ユライ ニコチンセイ アセチルコリン ジュヨウタイ β2 サブユニット ヘンイ ラット ノ ジハツセイ ホッサ
• Degree grantor/type: 弘前大学
• Date Granted: 2015-09-30
• Date Granted (W3CDTF): 2015-09-30
• Dissertation Number: 甲第1901号
• Degree Type: 博士（医学）
• Conferring No. (Dissertation): 甲第1901号
• Text Language Code: eng
• Subject Heading: autosomal dominant nocturnal frontal lobe epilepsy (ADNFLE); nicotinic acetylcholine receptor (nAChR) mutation; β2-subunit; epilepsy; seizures; transgenic rat; 基礎医学
• NDC: 491.3
• Target Audience: 一般
• Note (General): type:Thesis; We generated a transgenic rat strain with a missense mutation in V286L (V286L-TG),in the gene encoding the neuronal nicotinic acetylcholine receptor β2 subunit(CHRNB2) found in patients with autosomal dominant nocturnal frontal lobe epilepsy(ADNFLE). To confirm that V286L-TG rats exhibit seizures similar to those observed in humans, gene expression patterns and behavioral phenotypes were analyzed. In situhybridization using a V286L Chrnb2-selective probe indicated that the transgene was expressed at higher levels in the cortex, hippocampus, and cerebellum of V286L-TG than wild-type littermates (non-TG). Spontaneous epileptic seizures with ictal discharges in electroencephalograms were detected in 45% of V286L-TG rats and thefrequency of seizures was 0.73 times a week. This seizure type is similar to “paroxysmal arousals” that are observed in human ADNFLE. V286L-TG rats displayed nicotine-induced abnormal motor activity including seizures in comparison to non-TGs. Response time following nicotine administration occurred faster in V286L-TG than innon-TG rats. V286L-TG rats demonstrated spontaneous epileptic seizures, which are similar to human ADNFLE, and also showed a higher sensitivity to nicotine administration. Thus, the V286L-TG rat model could be a valuable tool for developing novel mechanism-driven treatment strategies for epilepsy and provide a better understanding of ADNFLE.; 掲載誌：Neurosci Res 100,p.46-54,2015
• DOI: info:doi/10.1016/j.neures.2015.06.003; https://doi.org/info:doi/10.1016/j.neures.2015.06.003
• Persistent ID (NDL): info:ndljp/pid/11122486; https://dl.ndl.go.jp/pid/11122486
• Collection: 障害者向け資料
• Collection (Materials For Handicapped People:1): テキストデータ
• Collection (particular): 国立国会図書館デジタルコレクション > デジタル化資料 > 博士論文; https://dl.ndl.go.jp/collections/A00014
• Acquisition Basis: 博士論文（自動収集）
• Date Accepted (W3CDTF): 2018-08-03T23:36:01+09:00
• Date Created (W3CDTF): 2018-03-13
• Format (IMT): application/pdf
• Access Restrictions: 国立国会図書館内限定公開
• Service for the Digitized Contents Transmission Service: 図書館・個人送信対象外
• Availability of remote photoduplication service: 可
• Periodical Title (URI): http://hdl.handle.net/10129/5662
• Data Provider (Database): 国立国会図書館 : 国立国会図書館デジタルコレクション; https://dl.ndl.go.jp