Porcine Dental Epithelial Cells Differentiated in a Cell Sheet Constructed by Magnetic Nanotechnology

• Material Type: 博士論文
• Title: Porcine Dental Epithelial Cells Differentiated in a Cell Sheet Constructed by Magnetic Nanotechnology
• Author/Editor: 古藤, 航
• Author Heading: 古藤, 航
• Publication Date: 2018-03-20
• Publication Date (W3CDTF): 2018-03-20
• Alternative Title: 磁性ナノ技術により作製した細胞シート中でブタ歯胚上皮細胞は分化する
• Contributor: 野中, 和明; 和田, 尚久; 築山, 能大
• Degree grantor/type: 九州大学
• Date Granted: 2018-03-20
• Date Granted (W3CDTF): 2018-03-20
• Dissertation Number: 甲第13981号
• Degree Type: 博士（歯学）
• Conferring No. (Dissertation): 甲第13981号
• Text Language Code: eng
• Target Audience: 一般
• Note (General): Magnetic nanoparticles (MNPs) are widely used in medical examinations, treatments, and basic research, including magnetic resonance imaging, drug delivery systems, and tissue engineering. In this study, MNPs with magnetic force were applied to tissue engineering for dental enamel regeneration. The internalization of MNPs into the odontogenic cells was observed by transmission electron microscopy. A combined cell sheet consisting of dental epithelial cells (DECs) and dental mesenchymal cells (DMCs) (CC sheet) was constructed using magnetic force-based tissue engineering technology. The result of the iron staining indicated that MNPs were distributed ubiquitously over the CC sheet. mRNA expression of enamel differentiation and basement membrane markers was examined in the CC sheet. Immunostaining showed Collagen IV expression at the border region between DEC and DMC layers in the CC sheet. These results revealed that epithelial–mesenchymal interactions between DEC and DMC layers were caused by bringing DECs close to DMCs mechanically by magnetic force. Our study suggests that the microenvironment in the CC sheet might be similar to that during the developmental stage of a tooth bud. In conclusion, a CC sheet employing MNPs could be developed as a novel and unique graft for artificially regenerating dental enamel.; PubMed番号 : 29027917
• DOI: 10.15017/1931834; https://doi.org/10.15017/1931834
• Persistent ID (NDL): info:ndljp/pid/11122697; https://dl.ndl.go.jp/pid/11122697
• Collection: 障害者向け資料
• Collection (Materials For Handicapped People:1): テキストデータ
• Collection (particular): 国立国会図書館デジタルコレクション > デジタル化資料 > 博士論文; https://dl.ndl.go.jp/collections/A00014
• Acquisition Basis: 博士論文（自動収集）
• Date Accepted (W3CDTF): 2018-08-03T23:36:01+09:00
• Date Created (W3CDTF): 2018-05-30
• Format (IMT): application/pdf
• Access Restrictions: 国立国会図書館内限定公開
• Service for the Digitized Contents Transmission Service: 図書館・個人送信対象外
• Availability of remote photoduplication service: 可
• Periodical Title (URI): http://dx.doi.org/10.15017/1931834; http://hdl.handle.net/2324/1931834
• Data Provider (Database): 国立国会図書館 : 国立国会図書館デジタルコレクション; https://dl.ndl.go.jp

• Summary, etc.: Magnetic nanoparticles (MNPs) are widely used in medical examinations, treatments, and basic research, including magnetic resonance imaging, drug deli...

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