Expression of neprilysin in periodontitis-affected gingival tissues

• Material Type: 博士論文
• Title: Expression of neprilysin in periodontitis-affected gingival tissues
• Author/Editor: Nezu, Arata
• Publication, Distribution, etc.: 新潟大学
• Publication Date: 2018-03-23
• Publication Date (W3CDTF): 2018-03-23
• Alternative Title: Expression of neprilysin in periodontitis-affected gingival tissues; 歯周炎罹患歯肉組織におけるネプリライシンの発現
• Degree grantor/type: 新潟大学
• Date Granted: 2018-03-23
• Date Granted (W3CDTF): 2018-03-23
• Dissertation Number: 甲第4439号
• Degree Type: 博士（歯学）
• Conferring No. (Dissertation): 甲第4439号
• Text Language Code: eng
• Subject Heading: Gene expression; Immunolocalisation; Periodontitis; Alzheimer’s disease; Amyloid beta precursor protein; Neprilysin
• Note (General): Objective: Although the pathogeneses of Alzheimer’s disease (AD) and periodontal diseases have overlapping features, including ageing and chronic inflammation, the association between AD and periodontitis remains unclear. To explore the pathogenesis of periodontitis, a comprehensive gene expression/transcriptome analysis in periodontitis-affected gingival tissues found that the AD pathway was significantly up-regulated in periodontitis-affected gingival tissues. AD-related genes, amyloid beta precursor protein (APP), interleukin-1 beta and compliment 1QA, were significantly elevated in periodontitis. In the present study, balance between mRNA expression of APP and a potent amyloid degradation enzyme, neprilysin (NEP), as well as protein localisation of APP and NEP were analysed. Design: Eighteen periodontitis-affected and 18 clinically healthy control gingival tissues were taken from patients with severe chronic periodontitis or undergoing tooth extraction. Total RNA was purified and used for quantitative reverse transcription real-time polymerase chain reaction (qRT-PCR). The localisation of APP and NEP was analysed by immunohistochemistry (IHC). Results: Both APP and NEP genes were up-regulated in periodontitis-affected gingival tissues. APP- expressing macrophages and NEP-expressing neutrophils and fibroblasts, reflecting inflammatory stages, were detected in inflamed gingival tissues by IHC. Conclusion: The up-regulation of APP and NEP mRNA levels in periodontitis-affected gingival tissues compared with healthy controls was confirmed by qRT-PCR analyses. Since NEP is one of the primary enzymes that degrades amyloid beta, increased NEP mRNA levels in periodontitis may act as an inhibitor of amyloid beta accumulation in gingival tissues, balancing increased APP mRNA expression. However, NEP has several effects including degradation of vasoactive substances; therefore, further sresearch is needed.; 学位の種類: 博士（歯学）. 報告番号: 甲第4439号. 学位記番号: 新大院博（歯）甲第402号. 学位授与年月日: 平成30年3月23日; Archives of Oral Biology. 2017, 79, 35-41.; 新大院博（歯）甲第402号; 元資料の権利情報 : Elsevier; 開始ページ : 35; 終了ページ : 41
• DOI: info:doi/10.1016/j.archoralbio.2017.03.003; https://doi.org/info:doi/10.1016/j.archoralbio.2017.03.003
• Persistent ID (NDL): info:ndljp/pid/11122888; https://dl.ndl.go.jp/pid/11122888
• Collection: 障害者向け資料
• Collection (Materials For Handicapped People:1): テキストデータ
• Collection (particular): 国立国会図書館デジタルコレクション > デジタル化資料 > 博士論文; https://dl.ndl.go.jp/collections/A00014
• Acquisition Basis: 博士論文（自動収集）
• Date Accepted (W3CDTF): 2018-08-03T23:36:01+09:00
• Date Created (W3CDTF): 2019-08-05
• Format (IMT): application/pdf
• Access Restrictions: 国立国会図書館内限定公開
• Service for the Digitized Contents Transmission Service: 図書館・個人送信対象外
• Availability of remote photoduplication service: 可
• Periodical Title (URI): http://hdl.handle.net/10191/50264
• Data Provider (Database): 国立国会図書館 : 国立国会図書館デジタルコレクション; https://dl.ndl.go.jp