The Role of Mutation Rates of GNAQ or GNA11 in Cases of Uveal Melanoma in Japan

• Material Type: 博士論文
• Title: The Role of Mutation Rates of GNAQ or GNA11 in Cases of Uveal Melanoma in Japan
• Author/Editor: Ominato, Jun
• Publication, Distribution, etc.: 新潟大学
• Publication Date: 2018-03-23
• Publication Date (W3CDTF): 2018-03-23
• Alternative Title: The Role of Mutation Rates of GNAQ or GNA11 in Cases of Uveal Melanoma in Japan; 日本人ぶどう膜悪性黒色腫症例におけるGNAQ遺伝子及びGNA11遺伝子の変異率と遺伝子変異と臨床病理学的所見との関連についての検討
• Degree grantor/type: 新潟大学
• Date Granted: 2018-03-23
• Date Granted (W3CDTF): 2018-03-23
• Dissertation Number: 甲第4390号
• Degree Type: 博士（医学）
• Conferring No. (Dissertation): 甲第4390号
• Text Language Code: eng
• Subject Heading: uveal melanoma; GNAQ/11 mutation; cell types; Ki-67
• Note (General): GNAQ and GNA11 mutations are thought to be important for the tumorigenesis of uveal melanoma. Although previous studies have reported on mutation rates in cases of uveal melanoma, presently, no such report for the Japanese population exists. In this study, we examined the frequency of GNAQ and GNA11 somatic mutations in cases of uveal melanoma in Japan and their relationship with clinicopathologic features or Ki-67-positive cell rates (Ki-67 labelingindex: Ki-67 LI) using immunofluorescence methods. The study involved 19 cases of uveal melanoma. We extracted the template DNA from formalin-fixed, paraffin-embedded specimens using a DNA extraction kit. We amplified the DNA sequences of GNAQ and GNA11 using polymerase chain reaction and analyzed mutations by direct sequencing. We evaluated Ki-67 LI using immunofluorescence methods. The frequencies of GNAQ and GNA11 somatic mutations were 26.3% (5/19) and 31.6% (6/19), respectively. The GNAQ and GNA11 mutations were mutually exclusive, as indicated in previous reports. The frequency of GNA11 mutations was significantly higher in epithelioid cells; however, no significant association between GNAQ mutations and cell type was evident, and there was no significant difference in Ki-67 LI between the mutation-positive and mutation-negative tumors. GNAQ and GNA11 mutations were identified in cases of uveal melanoma in Japan, although at lower frequencies than in white counterparts. The mutation frequency of GNA11 was significantly higher in epithelioid cells.; 学位の種類: 博士（医学）. 報告番号: 甲第4390号. 学位記番号: 新大院博（医）甲第789号. 学位授与年月日: 平成30年3月23日; Applied Immunohistochemistry & Molecular Morphology. 2017 . Publish Ahead of Print.; 新大院博（医）甲第789号; 開始ページ : 1; 終了ページ : 6
• DOI: info:doi/10.1097/PAI.0000000000000505; https://doi.org/info:doi/10.1097/PAI.0000000000000505
• Persistent ID (NDL): info:ndljp/pid/11123968; https://dl.ndl.go.jp/pid/11123968
• Collection: 障害者向け資料
• Collection (Materials For Handicapped People:1): テキストデータ
• Collection (particular): 国立国会図書館デジタルコレクション > デジタル化資料 > 博士論文; https://dl.ndl.go.jp/collections/A00014
• Acquisition Basis: 博士論文（自動収集）
• Date Accepted (W3CDTF): 2018-08-03T23:36:01+09:00
• Date Created (W3CDTF): 2019-08-05
• Format (IMT): application/pdf
• Access Restrictions: 国立国会図書館内限定公開
• Service for the Digitized Contents Transmission Service: 図書館・個人送信対象外
• Availability of remote photoduplication service: 可
• Periodical Title (URI): http://hdl.handle.net/10191/50415
• Data Provider (Database): 国立国会図書館 : 国立国会図書館デジタルコレクション; https://dl.ndl.go.jp