Nucleolin facilitates nuclear retention of an ultraconserved region containing TRA2β4 and accelerates colon cancer cell growth

• Material Type: 博士論文
• ISSN: 1949-2553
• Title: Nucleolin facilitates nuclear retention of an ultraconserved region containing TRA2β4 and accelerates colon cancer cell growth
• Volume: 9 42
• Author/Editor: Satake, Yuzuru; Kuwano, Yuki; Nishikawa, Tatsuya; Fujita, Kinuyo; Saijo, Saki; Itai, Miki; Tanaka, Hiroki; Nishida, Kensei; Rokutan, Kazuhito
• Author Heading: Satake, Yuzuru; Kuwano, Yuki; Nishikawa, Tatsuya; Fujita, Kinuyo; Saijo, Saki; Itai, Miki; Tanaka, Hiroki; Nishida, Kensei; Rokutan, Kazuhito
• Publication, Distribution, etc.: Impact Journals
• Publication Date: 2018-06-01
• Publication Date (W3CDTF): 2018-06-01
• Alternative Title: ヌクレオリンは、超保存領域を含むTRA2β4を核内に保持し、大腸がん細胞の増殖を促進する
• Periodical title: Oncotarget
• Degree grantor/type: 徳島大学
• Date Granted: 2018-07-26
• Date Granted (W3CDTF): 2018-07-26
• Dissertation Number: 甲第3201号
• Degree Type: 博士（医学）
• Conferring No. (Dissertation): 甲第3201号
• Text Language Code: eng
• Subject Heading: nucleolin; transcribed-UCR; TRA2β4; colon cancer; cell growth
• Target Audience: 一般
• Note (General): Transcribed-ultraconserved regions (T-UCRs), which contain conserved sequences with 100% identity across human, rat and mouse species, are a novel category of functional RNAs. The human transformer 2β gene (TRA2B) encodes a UCR that spans exon 2 (276 bp) and its neighboring introns. Among five spliced RNA variants (TRA2β1-5) transcribed from the TRA2B gene, only TRA2β4 contains the conserved exon 2. TRA2β4 is overexpressed in colon cancer cells and accelerates cell growth by blocking the transcription of CDKN1A. However, the mechanisms underlying the overexpression of TRA2β4 in colon cancer cells are unknown. Using biotinylated RNA pull-down assays followed by liquid chromatography-mass spectrometric analysis, we identified nucleolin as a TRA2β4-binding protein. Knockdown of nucleolin reduced the nuclear retention of TRA2β4 and accelerated its degradation in the cytoplasm, whereas nucleolin overexpression increased TRA2β4 levels and its mitogenic activity. Nucleolin directly bound to TRA2β4 exon 2 via the glycine/arginine-rich (GAR) domain. Overexpression of GAR-deficient nucleolin failed to increase TRA2β4 expression and growth of colon cancer cells. RNA fluorescence in situ hybridization showed that TRA2β4 co-localized with nucleolin in nuclei but not with the mutant lacking GAR. Our results suggest that specific interactions between nucleolin and UCR-containing TRA2β4 may be associated with abnormal growth of colon cancer cells.
• DOI: info:doi/10.18632/oncotarget.25510; https://doi.org/info:doi/10.18632/oncotarget.25510
• Persistent ID (NDL): info:ndljp/pid/11150997; https://dl.ndl.go.jp/pid/11150997
• Collection: 障害者向け資料
• Collection (Materials For Handicapped People:1): テキストデータ
• Collection (particular): 国立国会図書館デジタルコレクション > デジタル化資料 > 博士論文; https://dl.ndl.go.jp/collections/A00014
• Acquisition Basis: 博士論文（自動収集）
• Date Accepted (W3CDTF): 2018-09-03T21:27:21+09:00
• Format (IMT): application/pdf
• Access Restrictions: 国立国会図書館内限定公開
• Service for the Digitized Contents Transmission Service: 図書館・個人送信対象外
• Availability of remote photoduplication service: 可
• Periodical Title (URI): http://repo.lib.tokushima-u.ac.jp/112030
• Data Provider (Database): 国立国会図書館 : 国立国会図書館デジタルコレクション; https://dl.ndl.go.jp