Involvement of PUF60 in transcriptional and post-transcriptional regulation of hepatitis B virus pregenomic RNA expression

• Material Type: 博士論文
• ISSN: 2045-2322
• Title: Involvement of PUF60 in transcriptional and post-transcriptional regulation of hepatitis B virus pregenomic RNA expression
• Volume: 7
• Author/Editor: 孫, 鎖鋒
• Author Heading: 孫, 鎖鋒
• Publication, Distribution, etc.: Nature Publishing Group
• Publication Date: 2017-10-09
• Publication Date (W3CDTF): 2017-10-09
• Alternative Title: B型肝炎ウイルスプレゲノムRNA発現におけるPUF60による転写および転写後調節
• Periodical title: Scientific Reports
• Degree grantor/type: 浜松医科大学
• Date Granted: 2017-09-22
• Date Granted (W3CDTF): 2017-09-22
• Dissertation Number: 甲第765号
• Degree Type: 博士（医学）
• Conferring No. (Dissertation): 甲第765号
• Text Language Code: eng
• Target Audience: 一般
• Note (General): doctoral; 医学系研究科; Here we identified PUF60, a splicing factor and a U2 small nuclear ribonucleoprotein auxiliary factor, as a versatile regulator of transcriptional and post-transcriptional steps in expression of hepatitis B virus (HBV) 3.5 kb, precore plus pregenomic RNA. We demonstrate that PUF60 is involved in: 1) up-regulation of core promoter activity through its interaction with transcription factor TCF7L2, 2) promotion of 3.5 kb RNA degradation and 3) suppression of 3.5 kb RNA splicing. When the 1.24-fold HBV genome was introduced into cells with the PUF60-expression plasmid, the 3.5 kb RNA level was higher at days 1?2 post-transfection but declined thereafter in PUF60-expressing cells compared to viral replication control cells. Deletion analyses showed that the second and first RNA recognition motifs (RRMs) within PUF60 are responsible for core promoter activation and RNA degradation, respectively. Expression of PUF60 mutant deleting the first RRM led to higher HBV production. To our knowledge, this is the first to identify a host factor involved in not only positively regulating viral gene expression but also negative regulation of the same viral life cycle. Functional linkage between transcriptional and post-transcriptional controls during viral replication might be involved in mechanisms for intracellular antiviral defense and viral persistence.
• DOI: info:doi/10.1038/s41598-017-12497-y; https://doi.org/info:doi/10.1038/s41598-017-12497-y
• Persistent ID (NDL): info:ndljp/pid/11164082; https://dl.ndl.go.jp/pid/11164082
• Collection: 障害者向け資料
• Collection (Materials For Handicapped People:1): テキストデータ
• Collection (particular): 国立国会図書館デジタルコレクション > デジタル化資料 > 博士論文; https://dl.ndl.go.jp/collections/A00014
• Acquisition Basis: 博士論文（自動収集）
• Date Accepted (W3CDTF): 2018-10-03T17:18:55+09:00
• Date Created (W3CDTF): 2018-09-13
• Format (IMT): application/pdf
• Access Restrictions: 国立国会図書館内限定公開
• Service for the Digitized Contents Transmission Service: 図書館・個人送信対象外
• Availability of remote photoduplication service: 可
• Periodical Title (URI): http://hdl.handle.net/10271/00003397; https://hama-med.repo.nii.ac.jp/records/3252
• Data Provider (Database): 国立国会図書館 : 国立国会図書館デジタルコレクション; https://dl.ndl.go.jp

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