Genetically engineered multilineage-differentiating stress-enduring cells as cellular vehicles against malignant gliomas

• Material Type: 博士論文
• ISSN: 2372-7705
• Title: Genetically engineered multilineage-differentiating stress-enduring cells as cellular vehicles against malignant gliomas
• Volume: 6
• Author/Editor: 山﨑, 友裕
• Author Heading: 山﨑, 友裕
• Publication, Distribution, etc.: Elsevier
• Publication Date: 2017-09
• Publication Date (W3CDTF): 2017-09
• Alternative Title: 遺伝子改変Muse細胞は悪性グリオーマに対する細胞媒体となる
• Periodical title: Molecular therapy oncolytics
• Degree grantor/type: 浜松医科大学
• Date Granted: 2017-12-15
• Date Granted (W3CDTF): 2017-12-15
• Dissertation Number: 甲第766号
• Degree Type: 博士（医学）
• Conferring No. (Dissertation): 甲第766号
• Text Language Code: eng
• Target Audience: 一般
• Note (General): doctoral; 医学系研究科; Malignant glioma, the most common malignant brain tumor in adults, is difficult to treat due to its aggressive invasive nature. Enzyme/prodrug suicide gene therapy based on the herpes simplex virus thymidine kinase (HSVtk)/ganciclovir (GCV) system is an efficient strategy for treating malignant gliomas. In the present study, we evaluated treatment with multilineage-differentiating stress-enduring (Muse) cells, which are endogenous non-tumorigenic pluripotent-like stem cells that are easily collectable from the bone marrow as SSEA-3+ cells, as carriers of the HSVtk gene. Human Muse cells showed potent migratory activity toward glioma cells both in vitro and in vivo. HSVtk gene-transduced Muse cells (Muse-tk cells) at a cell number of only 1/32 that of U87 human glioma cells completely eradicated U87 gliomas in nude mouse brains, showing a robust in vivo bystander effect. Pre-existing intracranial U87 gliomas in nude mouse brains injected intratumorally with Muse-tk cells followed by intraperitoneal GCV administration were significantly reduced in size within 2 weeks, and 4 of 10 treated mice survived over 200 days. These findings suggest that intratumoral Muse-tk cell injection followed by systemic GCV administration is safe and effective and that allogeneic Muse-tk cell-medicated suicide gene therapy for malignant glioma is clinically feasible.
• DOI: info:doi/10.1016/j.omto.2017.06.001; https://doi.org/info:doi/10.1016/j.omto.2017.06.001
• Persistent ID (NDL): info:ndljp/pid/11195167; https://dl.ndl.go.jp/pid/11195167
• Collection: 障害者向け資料
• Collection (Materials For Handicapped People:1): テキストデータ
• Collection (particular): 国立国会図書館デジタルコレクション > デジタル化資料 > 博士論文; https://dl.ndl.go.jp/collections/A00014
• Acquisition Basis: 博士論文（自動収集）
• Date Accepted (W3CDTF): 2018-12-03T16:15:19+09:00
• Date Created (W3CDTF): 2018-10-26
• Format (IMT): application/pdf
• Access Restrictions: 国立国会図書館内限定公開
• Service for the Digitized Contents Transmission Service: 図書館・個人送信対象外
• Availability of remote photoduplication service: 可
• Periodical Title (URI): http://hdl.handle.net/10271/00003440; https://hama-med.repo.nii.ac.jp/records/3297
• Data Provider (Database): 国立国会図書館 : 国立国会図書館デジタルコレクション; https://dl.ndl.go.jp

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2023-12-09 再収集