Distinct Gene Expression Profile Distinguishes Increased Metabolic Activity in Spontaneously Hyperactive Rats While Sedentary from That Induced by Exercise

• Material Type: 博士論文
• ISSN: 2162-2191
• Title: Distinct Gene Expression Profile Distinguishes Increased Metabolic Activity in Spontaneously Hyperactive Rats While Sedentary from That Induced by Exercise
• Volume: 8 1
• Author/Editor: Abe, Manami; Matsuo, Yuki; Harada-Sukeno, Akiko; Uchida, Takayuki; Kitahata, Kanako; Tomida, Chisato; Hirasaka, Katsuya; Teshima-Kondo, Shigetada; Harada, Nagakatsu; Nakaya, Yutaka; Sakaue, Hiroshi; Nakao, Reiko; Nikawa, Takeshi
• Author Heading: Abe, Manami; Matsuo, Yuki; Harada-Sukeno, Akiko; Uchida, Takayuki; Kitahata, Kanako; Tomida, Chisato; Hirasaka, Katsuya; Teshima-Kondo, Shigetada; Harada, Nagakatsu; Nakaya, Yutaka; Sakaue, Hiroshi; Nakao, Reiko; Nikawa, Takeshi
• Publication, Distribution, etc.: Scientific Research Publishing
• Publication Date: 2018-02-05
• Publication Date (W3CDTF): 2018-02-05
• Alternative Title: 自発的高運動ラットの安静時遺伝子発現パターンと野生型ラット運動時遺伝子発現パターンの違い
• Periodical title: Advances in Biological Chemistry
• Degree grantor/type: 徳島大学
• Date Granted: 2018-03-15
• Date Granted (W3CDTF): 2018-03-15
• Dissertation Number: 甲第3145号
• Degree Type: 博士（栄養学）
• Conferring No. (Dissertation): 甲第3145号
• Text Language Code: eng
• Subject Heading: mitochondria; myofiber type; microarray; metabolism
• Target Audience: 一般
• Note (General): The Spontaneously-Running Tokushima Shikoku (SPORTS) strain is an original line derived from Wistar rats, which spontaneously runs >6 km/day on wheels, and has better glucose tolerance and less fat than Wistar rats. However, the molecular mechanism that contributes to the increased metabolic activity in SPORTS rats is unknown. The present study aimed to characterize the gene expression profiles of skeletal muscles in SPORTS rats housed under sedentary (SED) conditions. We found that the expression levels of genes encoding mitochondrial respiratory chain enzymes such as ATP synthase 6 (mt-Atp6) and cytochrome c oxidase subunit 6c (Cox 6c), were higher in the soleus (SOL) muscles of SED SPORTS than in SED Wistar rats. The ratio of type IIa myofibers was higher and glucose tolerance was better in SED SPORTS than in Wistar rats that were sedentary and trained daily on treadmills, respectively. We then investigated candidate genes that might contribute to the better glucose tolerance of SED SPORTS rats using DNA microarray analysis. Among 116 upregulated genes in the SOL muscles of SED SPORTS rats, only 19 were also increased in trained Wistar rats. We focused on v-erb-b2 erythroblastic leukemia viral oncogene homolog 3 (Erbb3), which was associated with glucose transport in myocytes, and found higher expression levels in the SOL muscles of SED SPORTS than in SED Wistar rats. The SOL muscles of SED SPORTS rats also contained more activity of β-hydroxy-acylCoA dehydrogenase, a key enzyme of β-oxidation, indicating enhanced lipid oxidation. These findings suggest that increased metabolic activity in skeletal muscle (especially the SOL muscle) of SPORTS rats is congenital and that gene expression profiles of SPORTS rats and Trained Wistar rats are different.
• DOI: info:doi/10.4236/abc.2018.81001; https://doi.org/info:doi/10.4236/abc.2018.81001
• Persistent ID (NDL): info:ndljp/pid/11216960; https://dl.ndl.go.jp/pid/11216960
• Collection: 障害者向け資料
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• Collection (particular): 国立国会図書館デジタルコレクション > デジタル化資料 > 博士論文; https://dl.ndl.go.jp/collections/A00014
• Acquisition Basis: 博士論文（自動収集）
• Date Accepted (W3CDTF): 2019-01-04T08:16:43+09:00
• Format (IMT): application/pdf
• Access Restrictions: 国立国会図書館内限定公開
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• Periodical Title (URI): http://repo.lib.tokushima-u.ac.jp/111609
• Data Provider (Database): 国立国会図書館 : 国立国会図書館デジタルコレクション; https://dl.ndl.go.jp