Ochratoxin A, citrinin and deoxynivalenol decrease claudin-2 expression in mouse rectum CMT93-II cells.

• Material Type: 博士論文
• Title: Ochratoxin A, citrinin and deoxynivalenol decrease claudin-2 expression in mouse rectum CMT93-II cells.
• Author/Editor: Nakayama, Hideaki; Kitagawa, Norio; Otani, Takahito; Iida, Hiroshi; Anan, Hisashi; Inai, Tetsuichiro
• Author Heading: Nakayama, Hideaki; Kitagawa, Norio; Otani, Takahito; Iida, Hiroshi; Anan, Hisashi; Inai, Tetsuichiro
• Publication Date: 2018-03-10
• Publication Date (W3CDTF): 2018-03-10
• Alternative Title: Ochratoxin A, citrinin and deoxynivalenol decrease claudin-2 expression in mouse rectum CMT93-II cells.
• Degree grantor/type: 福岡歯科大学
• Date Granted: 2018-03-10
• Date Granted (W3CDTF): 2018-03-10
• Dissertation Number: 甲第293号
• Degree Type: 博士（歯学）
• Conferring No. (Dissertation): 甲第293号
• Text Language Code: eng
• Subject Heading: Animals; Aspergillus ochraceus; Butadienes; Caco-2 Cells; Cell Line; Citrinin; Claudin-2; Dogs; Enzyme Inhibitors; Epithelial Cells; Fusarium; Gene Expression; Gene Expression Regulation; Humans; Intestinal Mucosa; Madin Darby Canine Kidney Cells; Mice; Mitogen-Activated Protein Kinases; Nitriles; Ochratoxins; Penicillium; Permeability; Phosphorylation; Rectum; Trichothecenes; tight junction; claudin; paracellular permeability; mycotoxin
• Target Audience: 一般
• Note (General): Intestinal epithelial cells are the first targets of ingested mycotoxins, such as ochratoxin A, citrinin and deoxynivalenol. It has been reported that paracellular permeability regulated by tight junctions is modulated by several mycotoxins by reducing the expression of specific claudins and integral membrane proteins in cell-cell contacts, accompanied by increase in phosphorylation of mitogen-activated protein kinases, including extracellular signal-related kinase (ERK) 1/2, p38 and c-Jun NH2-terminal protein kinase. Claudin-2 is expressed in the deep crypt cells, but not in the villus/surface cells in vivo. While Caco-2, T84 and IPEC-J2 cells, which are widely used intestinal epithelial cell lines to assess the influence of mycotoxins, do not express claudin-2, CMT93-II cells express claudin-2. We previously reported that inhibition of the ERK pathway reduced claudin-2 levels in cell-cell contacts in CMT93-II cells. In this study, we examined whether ochratoxin A, citrinin and deoxynivalenol affect claudin-2 expression and ERK1/2 phosphorylation in CMT93-II cells. We found that all mycotoxins reduced claudin-2 expression in cell-cell contacts, with reduction (by citrinin and deoxynivalenol) or no change (by ochratoxin A) in phosphorylated ERK1/2. All mycotoxins increased transepithelial electrical resistance, but did not affect flux of fluorescein. While ochratoxin A and citrinin are known to be nephrotoxic, only deoxynivalenol reduced claudin-2 expression in MDCK II cells derived from the renal tubule. These results suggest that claudin-2 expression is regulated not only by the ERK pathway, but also by other pathways in an organ-specific manner.; OXFORD
• Persistent ID (NDL): info:ndljp/pid/11246153; https://dl.ndl.go.jp/pid/11246153
• Collection: 障害者向け資料
• Collection (Materials For Handicapped People:1): テキストデータ
• Collection (particular): 国立国会図書館デジタルコレクション > デジタル化資料 > 博士論文; https://dl.ndl.go.jp/collections/A00014
• Acquisition Basis: 博士論文（自動収集）
• Date Accepted (W3CDTF): 2019-03-03T03:58:25+09:00
• Date Created (W3CDTF): 2019-02-04
• Format (IMT): application/pdf
• Access Restrictions: 国立国会図書館内限定公開
• Service for the Digitized Contents Transmission Service: 図書館・個人送信対象外
• Availability of remote photoduplication service: 可
• Original Material (URI): https://doi.org/10.1093/jmicro/dfy005
• Periodical Title (URI): https://fdc.repo.nii.ac.jp/records/91
• Data Provider (Database): 国立国会図書館 : 国立国会図書館デジタルコレクション; https://dl.ndl.go.jp

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