Lipopolysaccharide induces bacterial autophagy in epithelial keratinocytes of the gingival sulcus.

• Material Type: 博士論文
• Title: Lipopolysaccharide induces bacterial autophagy in epithelial keratinocytes of the gingival sulcus.
• Author/Editor: Kanako, Hagio-Izaki; Madoka, Yasunaga; Masahiro, Yamaguchi; Hiroshi, Kajiya; Hiromitsu, Morita; Masahiro, Yoneda; Takao, Hirofuji; Jun, Ohno
• Author Heading: Kanako, Hagio-Izaki; Madoka, Yasunaga; Masahiro, Yamaguchi; Hiroshi, Kajiya; Hiromitsu, Morita; Masahiro, Yoneda; Takao, Hirofuji; Jun, Ohno
• Publication Date: 2018-11-30
• Publication Date (W3CDTF): 2018-11-30
• Alternative Title: Lipopolysaccharide induces bacterial autophagy in epithelial keratinocytes of the gingival sulcus.
• Degree grantor/type: 福岡歯科大学
• Date Granted: 2018-11-30
• Date Granted (W3CDTF): 2018-11-30
• Dissertation Number: 甲第303号
• Degree Type: 博士（歯学）
• Conferring No. (Dissertation): 甲第303号
• Text Language Code: eng
• Subject Heading: 歯肉溝; オートファジー; ポルフィロモナスジンジバリス; リポポリサッカライド; 活性酸素; Gingival sulcus; Autophagy; Porphyromonas gingivalis; Lipopolysaccharide; Reactive oxygen species
• Target Audience: 一般
• Note (General): BACKGROUND:Interactions of resident bacteria and/or their producing lipopolysaccharide (LPS) with sulcular epithelial keratinocytes may be regulated by autophagy in the gingival sulcus. In this study, we investigated an induction of bacterial autophagy in exfoliative sulcular keratinocytes of the gingival sulcus and cultured keratinocytes treated with Porphyromonas gingivalis-originated LPS (PgLPS).; RESULTS:Exfoliative sulcular keratinocytes showed an induction of autophagy, in addition to increased expression of LPS-mediated factors including lipopolysaccharide-binding protein and toll-like receptors (TLRs), leading to co-localization of bacteria with autophagosomes. In contrast, exfoliative keratinocytes from the free gingiva did not show similar autophagy. Autophagy activity in human cultured keratinocyte cells (HaCaT) was induced by PgLPS, which was dependent partially on the AMP-activated protein kinase (AMPK) pathway via increased intracellular reactive oxygen species (ROS) and was in association with an activation of TLR4 signaling. After incubation of cultured keratinocytes with E.coli BioParticles following PgLPS stimulation, co-localization of bioparticles with autophagosomes was enhanced. Conversely, blockage of autophagy with 3-methyladenin and LPS-binding with polymyxin B led to significant reduction of co-localization of particles with autophagosomes.; CONCLUSION:These findings indicate that PgLPS-induced autophagy is at least partially responsible for interaction between bacteria and sulcular keratinocytes in the gingival sulcus.; BMC
• Persistent ID (NDL): info:ndljp/pid/11246158; https://dl.ndl.go.jp/pid/11246158
• Collection: 障害者向け資料
• Collection (Materials For Handicapped People:1): テキストデータ
• Collection (particular): 国立国会図書館デジタルコレクション > デジタル化資料 > 博士論文; https://dl.ndl.go.jp/collections/A00014
• Acquisition Basis: 博士論文（自動収集）
• Date Accepted (W3CDTF): 2019-03-03T03:58:25+09:00
• Date Created (W3CDTF): 2019-02-13
• Format (IMT): application/pdf
• Access Restrictions: 国立国会図書館内限定公開
• Service for the Digitized Contents Transmission Service: 図書館・個人送信対象外
• Availability of remote photoduplication service: 可
• Original Material (URI): https://dx.doi.org/10.1186%2Fs12860-018-0168-x
• Periodical Title (URI): https://fdc.repo.nii.ac.jp/records/99
• Data Provider (Database): 国立国会図書館 : 国立国会図書館デジタルコレクション; https://dl.ndl.go.jp

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