Predicting the tumorigenic phenotype of human bladder cancer cells by combining with fetal rat mesenchyme

• Material Type: 博士論文
• Title: Predicting the tumorigenic phenotype of human bladder cancer cells by combining with fetal rat mesenchyme
• Author/Editor: Miki, Manabu
• Author Heading: Miki, Manabu
• Publication, Distribution, etc.: 三重大学
• Publication Date: 2018-12-19
• Publication Date (W3CDTF): 2018-12-19
• Degree grantor/type: 三重大学
• Date Granted: 2018-12-19
• Date Granted (W3CDTF): 2018-12-19
• Dissertation Number: 甲医学第1926号
• Degree Type: 博士(医学)
• Conferring No. (Dissertation): 甲医学第1926号
• Text Language Code: jpn
• Alias of Author: ミキ, マナブ; 三木, 学
• Subject Heading: Invasive bladder cancer; Responsiveness of cancer cells to stroma; Fetal mesenchyme; Micropapillary-like structures; p63 isoforms Abbreviations; BLM,bladder mesenchyme; CIS,carcinoma in situ; EMT,epithelial-mesenchymal transition; H&E,hematoxylin and eosin; MIBC,muscle invasive bladder cancer; MVD,microvessel density; NMIBC,nonmuscle invasive bladder cancer; TUR,transurethral resection; UGM,urogenital sinus mesenchyme
• Target Audience: 一般
• Note (General): application/pdf; Background:In nonmuscle invasive bladder cancer patients, prediction of pTa and pT1 bladder cancer recurrence and progression must be established. Micropapillary structures have been defined as small clusters of invasive cancer cells having features of the epithelial-mesenchymal transition. Since the stromal microenvironment helps to induce the epithelial-mesenchymal transition, interactions between cancer cells and stroma should be closely examined to predict the tumorigenic phenotype of human bladder cancer cells.Materials and methods:To investigate differences in the responsiveness of cancer cells to stroma, we combined 3 established human bladder cancer cell lines (high-grade T24 and UM-UC-3 cells, and low-grade papillary RT4 cells) with fetal rat mesenchyme.Results:Among 3 bladder cancer cell lines, the expression profiles of p63 isoforms were distinct, i.e., p63γ in T24 cells, p63β in UM-UC-3 cells, and p63α in RT4 cells. Tumors formed by T24 cells combined with fetal mesenchyme formed micropapillary-like structures, whereas those formed by T24 cells alone did not. T24 cells combined with fetal mesenchyme showed poor differentiation, e.g., innumerable chromatic atypia in the nuclei, higher levels of chromatic condensation, and increased nucleoli. In contrast, both UM-UC-3 and RT4 cells combined with fetal mesenchyme did not form micropapillary-like structures. Ki-67 and p63 labeling indices were significantly elevated by combining fetal mesenchyme with T24 cells but not with the others.Conclusions:By mixing cancer cells with fetal mesenchyme, our data demonstrated that formation of micropapillary-like structures may predict the tumorigenic phenotype of invasive bladder cancer cells. Taken together, distinct expression profiles of p63 isoforms may predict poor outcomes in invasive bladder cancer.; 本文 / Department of Nephro-Urologic Surgery and Andrology, Mie University Graduate School of Medicine; 9p
• DOI: info:doi/10.1016/j.urolonc.2018.07.008; https://doi.org/info:doi/10.1016/j.urolonc.2018.07.008
• Persistent ID (NDL): info:ndljp/pid/11278920; https://dl.ndl.go.jp/pid/11278920
• Collection: 障害者向け資料
• Collection (Materials For Handicapped People:1): テキストデータ
• Collection (particular): 国立国会図書館デジタルコレクション > デジタル化資料 > 博士論文; https://dl.ndl.go.jp/collections/A00014
• Acquisition Basis: 博士論文（自動収集）
• Date Accepted (W3CDTF): 2019-05-06T10:27:56+09:00
• Date Created (W3CDTF): 2019-04-02
• Format (IMT): application/pdf
• Access Restrictions: 国立国会図書館内限定公開
• Service for the Digitized Contents Transmission Service: 図書館・個人送信対象外
• Availability of remote photoduplication service: 可
• Periodical Title (URI): http://hdl.handle.net/10076/00017961; https://mie-u.repo.nii.ac.jp/records/12329
• Data Provider (Database): 国立国会図書館 : 国立国会図書館デジタルコレクション; https://dl.ndl.go.jp

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2023-12-15 再収集