Peptidylarginine deiminase is involved in maintaining the cornified oral mucosa of rats.

• Material Type: 博士論文
• Title: Peptidylarginine deiminase is involved in maintaining the cornified oral mucosa of rats.
• Author/Editor: Arita, Seiichi; Hatta, Mitsutoki; Uchida, Kunitoshi; Kita, Tomo; Okamura, Kazuhiko; Ryu, Takanori; Murakami, Hiroshi; Sakagami, Ryuji; Yamazaki, Jun
• Author Heading: Arita, Seiichi; Hatta, Mitsutoki; Uchida, Kunitoshi; Kita, Tomo; Okamura, Kazuhiko; Ryu, Takanori; Murakami, Hiroshi; Sakagami, Ryuji; Yamazaki, Jun
• Publication Date: 2018-04-27
• Publication Date (W3CDTF): 2018-04-27
• Alternative Title: Peptidylarginine deiminase is involved in maintaining the cornified oral mucosa of rats.
• Degree grantor/type: 福岡歯科大学
• Date Granted: 2018-04-27
• Date Granted (W3CDTF): 2018-04-27
• Dissertation Number: 甲第300号
• Degree Type: 博士（歯学）
• Conferring No. (Dissertation): 甲第300号
• Text Language Code: eng
• Subject Heading: Animals; Animals, Newborn; Blotting, Western; Citrullination; Electrophoresis, Gel, Two-Dimensional; Fluorescent Antibody Technique; Intermediate Filament Proteins; Mouth Mucosa; Protein-Arginine Deiminases; RNA, Messenger; Rats; Rats, Wistar; Real-Time Polymerase Chain Reaction
• Target Audience: 一般
• Note (General): Epithelial cells derived from different regions exhibit marked differences in their differentiation capacity, allowing them to provide a suitable protective barrier. We aimed to clarify the role of peptidylarginine deiminase (PAD) in modifying the key epidermal proteins filaggrin (FLG) and keratin 1 (K1) during stratification of the rat palate and buccal mucosa.; We performed immunofluorescence, immunoblotting, PAD activity assays and 2-dimensional electrophoresis, and developed an organotypic culture model.; PAD1 expression was highest in the palate, whereas PAD2, PAD3 and PAD4 expression was highest in the skin, suggesting the tissue-specific expression of PAD isozymes that leads to differences in calcium dependency. Immunoblotting showed that the FLG monomer, as well as its degradation products and precursor (proFLG), were most abundantly expressed in the skin but had low expression in the palate, whereas only faint proFLG expression was detected in the buccal mucosa. FLG and K1 were colocalized with PAD1 and were likely to be citrullinated in the cornified layers of the skin; this colocalization was not detected on the palatal surface, and dot-like presence of proFLG that might be citrullinated and that of PAD1 were found in the granules of the palate. Organotypic models derived from the rat palate revealed that PAD inhibition reduced the breakdown of FLG, increased its association with K1 together with epithelial compaction, and decreased permeability in a dye permeability assay. Conversely, PAD stimulation had the opposite effects.; Citrullination is likely a protein modification that plays an important role in maintaining the structure and function of oral cornified mucosa in a way that is distinctly different from that of the skin.; WileyOnlineLibrary
• Persistent ID (NDL): info:ndljp/pid/11278994; https://dl.ndl.go.jp/pid/11278994
• Collection: 障害者向け資料
• Collection (Materials For Handicapped People:1): テキストデータ
• Collection (particular): 国立国会図書館デジタルコレクション > デジタル化資料 > 博士論文; https://dl.ndl.go.jp/collections/A00014
• Acquisition Basis: 博士論文（自動収集）
• Date Accepted (W3CDTF): 2019-05-06T10:27:56+09:00
• Date Created (W3CDTF): 2019-04-11
• Format (IMT): application/pdf
• Access Restrictions: 国立国会図書館内限定公開
• Service for the Digitized Contents Transmission Service: 図書館・個人送信対象外
• Availability of remote photoduplication service: 可
• Original Material (URI): https://onlinelibrary.wiley.com/doi/full/10.1111/jre.12561
• Periodical Title (URI): https://fdc.repo.nii.ac.jp/records/102
• Data Provider (Database): 国立国会図書館 : 国立国会図書館デジタルコレクション; https://dl.ndl.go.jp

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