黄色ブドウ球菌(Staphylococcus aureus) のBraRS 点変異によるナイシンA 高度耐性化

• Material Type: 博士論文
• Title: 黄色ブドウ球菌(Staphylococcus aureus) のBraRS 点変異によるナイシンA 高度耐性化
• Author/Editor: 有井, かおる
• Author Heading: 有井, かおる
• Publication, Distribution, etc.: John Wiley & Sons Ltd.
• Publication Date: 2019
• Publication Date (W3CDTF): 2019
• Alternative Title: Single mutations in BraRS confer high resistance against nisin A in Staphylococcus aureus.
• Periodical title: MicrobiologyOpen
• Degree grantor/type: 鹿児島大学
• Date Granted: 2019-03-25
• Date Granted (W3CDTF): 2019-03-25
• Dissertation Number: 甲総研第501号
• Degree Type: 博士(歯学); Doctor of Philosophy in Dental Science
• Conferring No. (Dissertation): 甲総研第501号
• Text Language Code: eng
• Subject Heading: ABC transporter; mutation; nisin A; resistance; two‐component system
• NDC: 497
• Target Audience: 一般
• Note (General): 博士論文全文, 博士論文要旨, 最終試験結果の要旨, 論文審査の要旨; Nisin A is a lantibiotic produced by Lactococcus lactis that is widely used as a food preservative. In Staphylococcus aureus, the BraRS two‐component system (TCS) senses nisin A and regulates the expression of the ABC transporter VraDE, which is responsible for nisin A resistance. In this study, we exposed S. aureus to a sub‐minimum inhibition concentration of nisin A and obtained three spontaneous mutants that were highly resistant to this lantibiotic, designated as SAN (S. aureus nisin resistant) 1, SAN8, and SAN87. In the wild‐type S. aureus strain, VraDE expression was induced by nisin A. In contrast, SAN8 and SAN87 showed constitutively high VraDE expression, even in the absence of nisin A, while SAN1 showed higher BraRS expression, which resulted in high VraDE expression in the presence of nisin A. We identified a single mutation in the promoter region of braXRS in SAN1, whereas SAN8 and SAN87 had single mutations in braR and braS, respectively. Interestingly, even the unphosphorylated form of the mutant BraR protein induced VraDE expression. These results indicate that conformational changes in BraS or BraR resulting from the point mutations may result in the constitutive expression of VraDE, allowing S. aureus to adapt to high concentrations of nisin A.
• DOI: info:doi/10.1002/mbo3.791; https://doi.org/info:doi/10.1002/mbo3.791
• Persistent ID (NDL): info:ndljp/pid/11279022; https://dl.ndl.go.jp/pid/11279022
• Collection: 障害者向け資料
• Collection (Materials For Handicapped People:1): テキストデータ
• Collection (particular): 国立国会図書館デジタルコレクション > デジタル化資料 > 博士論文; https://dl.ndl.go.jp/collections/A00014
• Acquisition Basis: 博士論文（自動収集）
• Date Accepted (W3CDTF): 2019-05-06T10:27:56+09:00
• Date Created (W3CDTF): 2019-04-17
• Format (IMT): PDF; application/pdf
• Access Restrictions: 国立国会図書館内限定公開
• Service for the Digitized Contents Transmission Service: 図書館・個人送信対象外
• Availability of remote photoduplication service: 可
• Original Material (URI): https://onlinelibrary.wiley.com/journal/20458827#pane-01cbe741-499a-4611-874e-1061f1f4679e01
• Periodical Title (URI): http://hdl.handle.net/10232/00030538
• Data Provider (Database): 国立国会図書館 : 国立国会図書館デジタルコレクション; https://dl.ndl.go.jp

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