Analysis of Bone Marrow-derived Mesenchymal Stem Cell Kinetics after Short-term Stimulation with Tumor Necrosis Factor-α (TNF-α)

• Material Type: 博士論文
• ISSN: 1341-7649
• Title: Analysis of Bone Marrow-derived Mesenchymal Stem Cell Kinetics after Short-term Stimulation with Tumor Necrosis Factor-α (TNF-α)
• Volume: 28 2
• Author/Editor: Naritani, Mio; Inoue, Miho; Raju, Resmi; Miyagi, Mayu; Oshima, Masamitsu; Matsuka, Yoshizo
• Author Heading: Naritani, Mio; Inoue, Miho; Raju, Resmi; Miyagi, Mayu; Oshima, Masamitsu; Matsuka, Yoshizo
• Publication, Distribution, etc.: THE SOCIETY FOR HARD TISSUE REGENERATIVE BIOLOGY
• Publication Date: 2019-04-24
• Publication Date (W3CDTF): 2019-04-24
• Alternative Title: TNF-α短期刺激による骨髄由来間葉系幹細胞の細胞動態解析; Effect of Short Term TNF-α Stimulation of BMSCs
• Periodical title: Journal of Hard Tissue Biology
• Degree grantor/type: 徳島大学
• Date Granted: 2019-03-22
• Date Granted (W3CDTF): 2019-03-22
• Dissertation Number: 甲第3282号
• Degree Type: 博士（歯学）
• Conferring No. (Dissertation): 甲第3282号
• Text Language Code: eng
• Subject Heading: TNF-α; Bone marrow-derived mesenchymal stem cells; Short-term stimulation; Cell proliferation; Cell differentiation
• Target Audience: 一般
• Note (General): Bone marrow-derived mesenchymal stem cells (BMSCs) have considerable potential for self-renewal and multi-differentiation. Tumor necrosis factor-α (TNF-α) is an inflammatory cytokine and is involved in tissue regeneration during wound healing. It was already reported that cultured human dental pulp cells acquire stem cell properties following short-term stimulation by TNF-α. However, it has not been clarified if BMSCs acquire stem cell properties after TNF-α treatment. The purpose of this study was to investigate the effect of short-term stimulation with TNF-α on BMSCs. Rat BMSCs were cultured up to 60% confluence and then incubated with 1–100 ng/ml of recombinant rat TNF-α (rTNF-α) for a further 2 days. After reaching subconfluence, cells were passaged once to remove rTNF-α completely before subsequent assays. Cells in the control group were passaged without stimulation. Expression levels of Nanog and Oct4 stem cell markers were significantly increased in the rTNF-α 10 ng/ml stimulation group. rTNF-α stimulation did not affect cell proliferation compared with the control group. However, rTNF-α stimulation led to a delay in cell differentiation. This study suggests that short-term TNF-α stimulation of BMSCs significantly increased their stem cell phenotype, but delayed their osteogenic cell differentiation.
• DOI: info:doi/10.2485/jhtb.28.99; https://doi.org/info:doi/10.2485/jhtb.28.99
• Persistent ID (NDL): info:ndljp/pid/11300551; https://dl.ndl.go.jp/pid/11300551
• Collection: 障害者向け資料
• Collection (Materials For Handicapped People:1): テキストデータ
• Collection (particular): 国立国会図書館デジタルコレクション > デジタル化資料 > 博士論文; https://dl.ndl.go.jp/collections/A00014
• Acquisition Basis: 博士論文（自動収集）
• Date Accepted (W3CDTF): 2019-07-05T01:13:02+09:00
• Format (IMT): application/pdf
• Access Restrictions: 国立国会図書館内限定公開
• Service for the Digitized Contents Transmission Service: 図書館・個人送信対象外
• Availability of remote photoduplication service: 可
• Periodical Title (URI): http://repo.lib.tokushima-u.ac.jp/113372
• Data Provider (Database): 国立国会図書館 : 国立国会図書館デジタルコレクション; https://dl.ndl.go.jp