GSK-3 inhibitor inhibits cell proliferation and induces apoptosis in human osteosarcoma cells

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DOI[info:doi/10.3892/or.2016.4565]to the data of the same series

GSK-3 inhibitor inhibits cell proliferation and induces apoptosis in human osteosarcoma cells

Persistent ID (NDL): info:ndljp/pid/11337526

Material type: 博士論文

Author: 西村, 英樹

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Publication date: 2016-03-24

Material Format: Digital

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Name of awarding university/degree: 香川大学,博士（医学）

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Note (General)：: AbstractGlycogen synthase kinase-3 (GSK-3) is a serine/threonine kinase that functions in numerous signaling pathways initiated by diverse stimuli. Th...

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Material Type: 博士論文
Title: GSK-3 inhibitor inhibits cell proliferation and induces apoptosis in human osteosarcoma cells
Author/Editor: 西村, 英樹
Author Heading: 西村, 英樹
Publication Date: 2016-03-24
Publication Date (W3CDTF): 2016-03-24
Degree grantor/type: 香川大学
Date Granted: 2016-03-24
Date Granted (W3CDTF): 2016-03-24
Dissertation Number: 甲第622号
Degree Type: 博士（医学）
Conferring No. (Dissertation): 甲第622号
Text Language Code: eng
Alias of Author: Nishimura, Hideki
Subject Heading: glycogen synthase kinase-3
SB216763
apoptosis
osteosarcoma
Target Audience: 一般
Note (General): AbstractGlycogen synthase kinase-3 (GSK-3) is a serine/threonine kinase that functions in numerous signaling pathways initiated by diverse stimuli. The functions of GSK-3 in cancer differ depending on cell type. In the present study, we examined the effects of a specific GSK-3 inhibitor on the regulation of osteosarcoma cell proliferation and apoptosis. Immunohistochemical analysis and real-time reverse transcription-polymerase chain reaction (RT-PCR) were performed to determine the expression pattern of GSK-3 in human osteosarcoma cells. We used the MTS assay, western blotting, measurement of single-stranded DNA and morphological analyses to study the effects of a GSK-3 inhibitor, SB216763 on osteosarcoma cell proliferation and survival. We detected an increase in mRNA expression of GSK-3 and aberrant nuclear accumulation of GSK-3 in the osteosarcoma cells. Pharmacological inhibition of GSK-3 led to a decrease in proliferation and survival of osteosarcoma cells. Inhibition of GSK-3 resulted in a decreased expression of Bcl-2 and a subsequent increase in osteosarcoma cell apoptosis via the mitochondrial pathway. The present study demonstrated that GSK-3 activity is critical for tumorigenicity and cell survival in osteosarcoma cells. Our findings suggest that GSK-3 is a potential therapeutic target for the treatment of human osteosarcoma.
DOI: info:doi/10.3892/or.2016.4565
https://doi.org/info:doi/10.3892/or.2016.4565
Persistent ID (NDL): info:ndljp/pid/11337526
https://dl.ndl.go.jp/pid/11337526
Collection (particular): 国立国会図書館デジタルコレクション > デジタル化資料 > 博士論文
https://dl.ndl.go.jp/collections/A00014
Acquisition Basis: 博士論文（自動収集）
Date Accepted (W3CDTF): 2019-08-03T12:23:27+09:00
Date Created (W3CDTF): 2020-10-27
Format (IMT): PDF
Access Restrictions: 国立国会図書館内限定公開
Service for the Digitized Contents Transmission Service: 図書館・個人送信対象外
Availability of remote photoduplication service: 可
Periodical Title (URI): https://kagawa-u.repo.nii.ac.jp/records/376
Data Provider (Database): 国立国会図書館 : 国立国会図書館デジタルコレクション
https://dl.ndl.go.jp