Inhibitory effect of isoamericanol A from Jatropha curcas seeds on the growth of MCF-7 human breast cancer cell line by G2/M cell cycle arrest

Persistent ID (NDL): info:ndljp/pid/11337528

Material type: 博士論文

Author: 片木, 絢子

Publisher: -

Publication date: 2016-03-24

Material Format: Digital

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Name of awarding university/degree: 香川大学,博士（医学）

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Note (General)：: AbstractAlthough various parts of J. curcas (Jatropha curcas L., Euphorbiaceae) have long been used as traditional folk medicines for their antiviral,...

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Material Type: 博士論文
Title: Inhibitory effect of isoamericanol A from Jatropha curcas seeds on the growth of MCF-7 human breast cancer cell line by G2/M cell cycle arrest
Author/Editor: 片木, 絢子
Author Heading: 片木, 絢子
Publication Date: 2016-03-24
Publication Date (W3CDTF): 2016-03-24
Degree grantor/type: 香川大学
Date Granted: 2016-03-24
Date Granted (W3CDTF): 2016-03-24
Dissertation Number: 甲第624号
Degree Type: 博士（医学）
Conferring No. (Dissertation): 甲第624号
Text Language Code: eng
Alias of Author: Katagi, Ayako
Subject Heading: Health sciences
Pharmaceutical science
Plant biology
Cancer treatment
Cancer
Target Audience: 一般
Note (General): AbstractAlthough various parts of J. curcas (Jatropha curcas L., Euphorbiaceae) have long been used as traditional folk medicines for their antiviral, analgesic, and/or antidotal efficacies, we are the first to investigate the role of anti-carcinogenicity of isoamericanol A (IAA) from the seed extract. Our results showed that IAA is capable of inhibiting cell proliferation in a dose-dependent manner on the human cancer cell lines of MCF-7, MDA-MB231, HuH-7, and HeLa. Flow cytometry analysis showed IAA significantly induces cell cycle arrest at G2/M on MCF-7 cells. At both protein and mRNA levels examined by western blot and real-time PCR, the results revealed increased expression of BTG2 (B-cell translocation gene 2), p21 (p21WAF1/CIPI), and GADD45A (growth arrest and DNA-damage-inducible, alpha) after IAA treatment, but inversed expression in CDK1 (cyclin-dependent kinase 1) and cyclins B1 and B2. All these effects contribute to G2/M cell cycle arrest. Furthermore, these results coincide with the changes in molecular expressions determined by DNA-microarray analysis. Our findings indicate that IAA has an inhibitory effect on cell proliferation of MCF-7 through cell cycle arrest, giving it great potential as a future therapeutic reagent for cancers.
Persistent ID (NDL): info:ndljp/pid/11337528
https://dl.ndl.go.jp/pid/11337528
Collection (particular): 国立国会図書館デジタルコレクション > デジタル化資料 > 博士論文
https://dl.ndl.go.jp/collections/A00014
Acquisition Basis: 博士論文（自動収集）
Date Accepted (W3CDTF): 2019-08-03T12:23:27+09:00
Date Created (W3CDTF): 2020-10-27
Format (IMT): PDF
Access Restrictions: 国立国会図書館内限定公開
Service for the Digitized Contents Transmission Service: 図書館・個人送信対象外
Availability of remote photoduplication service: 可
Original Material (URI): 10.1016%2Fj.heliyon.2015.e00055
Periodical Title (URI): https://kagawa-u.repo.nii.ac.jp/records/378
Data Provider (Database): 国立国会図書館 : 国立国会図書館デジタルコレクション
https://dl.ndl.go.jp