Chloroquine Enhances Rapamycin-induced Apoptosis in MG63 Cells

Persistent ID (NDL): info:ndljp/pid/11346155

Material type: 博士論文

Author: 石橋, 洋一

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Publication date: 2019-06-25

Material Format: Digital

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Name of awarding university/degree: 香川大学,博士（医学）

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Note (General)：: Background/aim: We previously showed that the use of autophagy inhibitors in combination with chemotherapy can enhance anticancer effects in sarcoma c...

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Material Type: 博士論文
Title: Chloroquine Enhances Rapamycin-induced Apoptosis in MG63 Cells
Author/Editor: 石橋, 洋一
Author Heading: 石橋, 洋一
Publication Date: 2019-06-25
Publication Date (W3CDTF): 2019-06-25
Degree grantor/type: 香川大学
Date Granted: 2019-06-25
Date Granted (W3CDTF): 2019-06-25
Dissertation Number: 甲第722号
Degree Type: 博士（医学）
Conferring No. (Dissertation): 甲第722号
Text Language Code: eng
Alias of Author: Ishibashi, Yoichi
Subject Heading: Rapamycin
chloroquine
autophagy
apoptosis
osteosarcoma
Target Audience: 一般
Note (General): Background/aim: We previously showed that the use of autophagy inhibitors in combination with chemotherapy can enhance anticancer effects in sarcoma cell lines. In this study, we investigated the combined effect of the autophagy inhibitor chloroquine and the mTOR inhibitor rapamycin on MG63 osteosarcoma cells.
Materials and methods: Effects of chloroquine and/or rapamycin on cell proliferation were assessed by WST-1 assays. Effects of chloroquine and/or rapamycin on the mTOR pathway components, autophagy, and apoptosis were investigated by western blot, flow cytometry, and fluorescence microscopy using immunocytochemical staining of LC3 and Annexin V-FITC/propidium iodide.
Results: Rapamycin suppressed cell growth and inhibited the mTOR pathway. Rapamycin promoted autophagy by blocking the mTOR pathway, and chloroquine enhanced apoptosis by blocking autophagy.
Conclusion: Chloroquine enhances the effects of rapamycin in inducing apoptosis via autophagy inhibition in MG63 cells. Thus, the combined therapy of chloroquine and rapamycin may be a potent treatment for osteosarcoma.
DOI: info:doi/10.21873/anticanres.13159
https://doi.org/info:doi/10.21873/anticanres.13159
Persistent ID (NDL): info:ndljp/pid/11346155
https://dl.ndl.go.jp/pid/11346155
Collection (particular): 国立国会図書館デジタルコレクション > デジタル化資料 > 博士論文
https://dl.ndl.go.jp/collections/A00014
Acquisition Basis: 博士論文（自動収集）
Date Accepted (W3CDTF): 2019-09-07T00:28:35+09:00
Date Created (W3CDTF): 2020-10-27
Format (IMT): PDF
Access Restrictions: 国立国会図書館内限定公開
Service for the Digitized Contents Transmission Service: 図書館・個人送信対象外
Availability of remote photoduplication service: 可
Periodical Title (URI): https://kagawa-u.repo.nii.ac.jp/records/398
Data Provider (Database): 国立国会図書館 : 国立国会図書館デジタルコレクション
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