TCR affinity for in vivo peptide-induced thymic positive selection finetunes TCR responsiveness of peripheral CD8+ T cells 4

• Material Type: 博士論文
• ISSN: 0022-1767
• Title: TCR affinity for in vivo peptide-induced thymic positive selection finetunes TCR responsiveness of peripheral CD8+ T cells
• Volume: 203 4
• Author/Editor: Khanom, Umme Shahina; Ohigashi, Izumi; Fujimori, Sayumi; Kondo, Kenta; Takada, Kensuke; Takahama, Yousuke
• Author Heading: Khanom, Umme Shahina; Ohigashi, Izumi; Fujimori, Sayumi; Kondo, Kenta; Takada, Kensuke; Takahama, Yousuke
• Publication, Distribution, etc.: The American Association of Immunologists, Inc.
• Publication Date: 2019-06-24
• Publication Date (W3CDTF): 2019-06-24
• Alternative Title: 胸腺の正の選択におけるTCR親和性は末梢CD8陽性T細胞のTCR応答性を微調整する; IN VIVO PEPTIDES POSITIVELY SELECT AND FINE-TUNE T CELLS
• Periodical title: The Journal of Immunology
• Degree grantor/type: 徳島大学
• Date Granted: 2019-07-25
• Date Granted (W3CDTF): 2019-07-25
• Dissertation Number: 甲第3323号
• Degree Type: 博士（医学）
• Conferring No. (Dissertation): 甲第3323号
• Text Language Code: eng
• Subject Heading: CD8+ T cells; Peptide; Positive selection; Affinity
• Target Audience: 一般
• Note (General): The affinity for TCR interactions with self-peptide/MHC complexes (pMHC) in the thymus critically affects immature thymocytes that newly express TCRs. Previous fetal thymus organ culture experiments have indicated that difference in the affinity for thymic TCR/pMHC interactions not only determines thymocyte fate between positive and negative selection, but also affects Ag responsiveness of positively selected thymocytes. In the current study, we examined whether TCR/pMHC affinity during positive selection in the thymus would further affect Ag responsiveness of mature T cells in the periphery. To do so, OVA peptide variants were in vivo administered to TAP1-deficient OT-I/TCR-transgenic mice in which T cell development was otherwise arrested at CD4+CD8+ thymocytes because of the lack of self-pMHC presentation in thymic APCs. We found that a group of peptide variants induced the transient generation of OT-I CD8+ T cells in the thymus and the periphery. We also noticed that the affinity threshold for positive and negative selection detected in adult mice in vivo was higher than that measured in fetal thymus organ culture experiments in vitro. Interestingly, we further found that the affinity for positively selecting peptides proportionally affected TCR responsiveness of peripheral naive CD8+ T cells. These results indicate that in vivo administration of a peptide can promote T cell selection in the thymus and the affinity for TCR/pMHC interaction during positive selection fine-tunes Ag responsiveness of peripheral T cells.
• DOI: info:doi/10.4049/jimmunol.1900097; https://doi.org/info:doi/10.4049/jimmunol.1900097
• Persistent ID (NDL): info:ndljp/pid/11361583; https://dl.ndl.go.jp/pid/11361583
• Collection: 障害者向け資料
• Collection (Materials For Handicapped People:1): テキストデータ
• Collection (particular): 国立国会図書館デジタルコレクション > デジタル化資料 > 博士論文; https://dl.ndl.go.jp/collections/A00014
• Acquisition Basis: 博士論文（自動収集）
• Date Accepted (W3CDTF): 2019-10-04T14:36:02+09:00
• Format (IMT): application/pdf
• Access Restrictions: 国立国会図書館内限定公開
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• Periodical Title (URI): http://repo.lib.tokushima-u.ac.jp/113696
• Data Provider (Database): 国立国会図書館 : 国立国会図書館デジタルコレクション; https://dl.ndl.go.jp