Note (General)Eco-friendly enzymatic treatment using keratinase is expected to degrade wool surface cuticle instead of traditional chloride treatment The study examined a keratinase from Meiothermus ruber H328 (Keratinase H328) on degrading wool keratin of cuticle. The optimum treatment condition of Keratinase H328 such as incubation time and enzyme concentration was found for wool sliver, yarn and fabric. A subsequent Proteinase K treatment was conducted to enhance the degradation of Keratinase H328 pretreated wool. Due to the hydrophilization of wool surface after removing cuticle with lipid layer, a method was introduced to resume the hydrophobic surface for wool fabric. The wool samples after various treatments were characterized by physicochemical method, KES-F surface, mechanical and thermal tests. Results showed Keratinase H328 eroded wool scale slightly and did little change on wool’s mechanical properties. Keratinase H328 mixture rather than Proteinase K can degrade the disulfide bonds in wool keratin. Combined Kerainase H328-Proteinase K treatment more considerably damaged wool cuticle than pure keratinase or protease treatment. An obvious increase of moisture regain was observed in enzyme treated sample, while maximum increase was found in combined enzymatic treatment, which could relate to the degree of damage on fiber surface. Larger qmax (the maximum heat flux) values were achieved in samples with higher moisture regain, suggesting the qmax is potential to characterize the enzymatic treatment. Furthermore, qmax was verified to relate the fabric surface properties such as surface roughness and fuzz. Besides, the grafting of poly (acrylic acid) and dodecyl amine transferred the hydrophilicity of scale-damaged wool surface into hydrophobicity. This study introduces a specific enzymatic treatment on wool using keratinase, a damage repair method and their characterization. It will support the mechanistic study of keratinase activity, and process improvement of keratinase treatment to minimize the harsh hard, stimuli perception of prickle and itch, and shrinking.
Collection (particular)国立国会図書館デジタルコレクション > デジタル化資料 > 博士論文
Date Accepted (W3CDTF)2019-10-04T14:36:02+09:00
Data Provider (Database)国立国会図書館 : 国立国会図書館デジタルコレクション