Epidermal growth factor receptor-targeted molecular imaging of colorectal tumors : Detection and treatment evaluation of tumors in animal models

• Material Type: 博士論文
• ISSN: 1349-7006
• Title: Epidermal growth factor receptor-targeted molecular imaging of colorectal tumors : Detection and treatment evaluation of tumors in animal models
• Volume: 110 6
• Author/Editor: Miyamoto, Yoshihiko; Muguruma, Naoki; Fujimoto, Shota; Okada, Yasuyuki; Kida, Yoshifumi; Nakamura, Fumika; Tanaka, Kumiko; Nakagawa, Tadahiko; Kitamura, Shinji; Okamoto, Koichi; Miyamoto, Hiroshi; Sato, Yasushi; Takayama, Tetsuji
• Author Heading: Miyamoto, Yoshihiko; Muguruma, Naoki; Fujimoto, Shota; Okada, Yasuyuki; Kida, Yoshifumi; Nakamura, Fumika; Tanaka, Kumiko; Nakagawa, Tadahiko; Kitamura, Shinji; Okamoto, Koichi; Miyamoto, Hiroshi; Sato, Yasushi; Takayama, Tetsuji
• Publication, Distribution, etc.: Wiley|Japanese Cancer Association
• Publication Date: 2019-04-11
• Publication Date (W3CDTF): 2019-04-11
• Alternative Title: 上皮成長因子受容体を標的とした結腸直腸腫瘍の分子イメージング : 動物モデルにおける腫瘍の検出と治療評価
• Periodical title: Cancer Science
• Degree grantor/type: 徳島大学
• Date Granted: 2019-09-26
• Date Granted (W3CDTF): 2019-09-26
• Dissertation Number: 甲第3333号
• Degree Type: 博士（医学）
• Conferring No. (Dissertation): 甲第3333号
• Text Language Code: eng
• Subject Heading: colorectal cancer; epidermal growth factor receptor; fluorescence endoscopy; molecular imaging; therapeutic efficacy
• Target Audience: 一般
• Note (General): To overcome the problem of overlooking colorectal tumors, a new and highly sensitive modality of colonoscopy is needed. Moreover, it is also important to establish a new modality to evaluate viable tumor volume in primary lesions of colorectal cancer (CRC) during chemotherapy. Therefore, we carried out molecular imaging of colorectal tumors targeting epidermal growth factor receptor (EGFR), which is highly expressed on tumor cells, for evaluating chemotherapeutic efficacy and for endoscopic detection of colorectal adenomas. We first attempted to image five CRC cell lines with various levels of EGFR expression using an Alexa Fluor‐labeled anti‐EGFR monoclonal antibody (AF‐EGFR‐Ab). A strong fluorescence signal was observed in the cells depending on the level of EGFR expression. When nude mice xenografted with LIM1215 CRC cells, which highly express EGFR, were i.v. injected with AF‐EGFR‐Ab, a strong fluorescence signal appeared in the tumor with a high signal to noise ratio, peaking at 48 hours after injection and then gradually decreasing, as shown using an IVIS Spectrum system. When the xenografted mice were treated with 5‐fluorouracil, fluorescence intensity in the tumor decreased in proportion to the viable tumor cell volume. Moreover, when the colorectum of azoxymethane‐treated rats was observed using a thin fluorescent endoscope with AF‐EGFR‐Ab, all 10 small colorectal adenomas (≤3 mm) were detected with a clear fluorescence signal. These preliminary results of animal experiments suggest that EGFR‐targeted fluorescent molecular imaging may be useful for quantitatively evaluating cell viability in CRC during chemotherapy, and also for detecting small adenomas using a fluorescent endoscope.
• DOI: info:doi/10.1111/cas.14020; https://doi.org/info:doi/10.1111/cas.14020
• Persistent ID (NDL): info:ndljp/pid/11378271; https://dl.ndl.go.jp/pid/11378271
• Collection: 障害者向け資料
• Collection (Materials For Handicapped People:1): テキストデータ
• Collection (particular): 国立国会図書館デジタルコレクション > デジタル化資料 > 博士論文; https://dl.ndl.go.jp/collections/A00014
• Acquisition Basis: 博士論文（自動収集）
• Date Accepted (W3CDTF): 2019-11-04T14:30:53+09:00
• Format (IMT): application/pdf
• Access Restrictions: 国立国会図書館内限定公開
• Service for the Digitized Contents Transmission Service: 図書館・個人送信対象外
• Availability of remote photoduplication service: 可
• Periodical Title (URI): http://repo.lib.tokushima-u.ac.jp/113790
• Data Provider (Database): 国立国会図書館 : 国立国会図書館デジタルコレクション; https://dl.ndl.go.jp