Eya2 is critical for the E2A-HLF-mediated immortalization of mouse hematopoietic stem/progenitor cells

• Material Type: 博士論文
• Title: Eya2 is critical for the E2A-HLF-mediated immortalization of mouse hematopoietic stem/progenitor cells
• Author/Editor: MAHARJAN, BISHNU DEVI
• Author Heading: MAHARJAN, BISHNU DEVI
• Publication, Distribution, etc.: 三重大学
• Publication Date: 2019-03-25
• Publication Date (W3CDTF): 2019-03-25
• Degree grantor/type: 三重大学
• Date Granted: 2019-03-25
• Date Granted (W3CDTF): 2019-03-25
• Dissertation Number: 甲医学第1953号
• Degree Type: 博士(医学)
• Conferring No. (Dissertation): 甲医学第1953号
• Text Language Code: eng
• Subject Heading: Eya2; E2A-HLF; leukemia; self-renewal; immortalization
• Target Audience: 一般
• Note (General): application/pdf; The E2A-HLF oncogenic fusion gene, generated by t(17;19)(q22;p13) translocation in childhood B-cell acute lymphoblastic leukemia with a very poor prognosis, encodes a chimeric transcription factor in which the transactivation domains of immunoglobulin enhancer-binding factor (E2A) are fused to the DNA-binding and dimerization domain of hepatic leukemia factor (HLF). E2A-HLF was shown to have an anti-apoptotic effect. However, the molecular mechanism underlying E2A-HLF-mediated leukemogenesis remains unclear. We identified the transcriptional coactivator Eya2, the forced expression of which is known to immortalize mouse hematopoietic stem/progenitor cells (HSPCs), as a direct target molecule downstream of E2A-HLF. E2A-HLF-immortalized mouse HSPCs expressed Eya2 at a high level in the aberrant self-renewal program. Chromatin immunoprecipitation-quantitative polymerase chain reaction and a reporter assay revealed that E2A-HLF enhanced the Eya2 expression by binding to the promoter region containing the E2A-HLF-binding consensus sequence. Eya2 knockdown in E2A-HLF-immortalized cells resulted in reduced colony-forming efficiency. These results suggest a critical role of Eya2 in E2A-HLF-mediated leukemogenesis.; 本文/Department of Microbiology and Molecular Genetics, Mie University Graduate School of Medicine,; 27p
• Persistent ID (NDL): info:ndljp/pid/11378289; https://dl.ndl.go.jp/pid/11378289
• Collection: 障害者向け資料
• Collection (Materials For Handicapped People:1): テキストデータ
• Collection (particular): 国立国会図書館デジタルコレクション > デジタル化資料 > 博士論文; https://dl.ndl.go.jp/collections/A00014
• Acquisition Basis: 博士論文（自動収集）
• Date Accepted (W3CDTF): 2019-11-04T14:30:53+09:00
• Date Created (W3CDTF): 2019-10-16
• Format (IMT): application/pdf
• Access Restrictions: 国立国会図書館内限定公開
• Service for the Digitized Contents Transmission Service: 図書館・個人送信対象外
• Availability of remote photoduplication service: 可
• Periodical Title (URI): http://hdl.handle.net/10076/00018411; https://mie-u.repo.nii.ac.jp/records/12792
• Data Provider (Database): 国立国会図書館 : 国立国会図書館デジタルコレクション; https://dl.ndl.go.jp

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2023-12-15 再収集