Effects of extracellular DNA and DNA-binding protein on the development of a Streptococcus intermedius biofilm

• Material Type: 博士論文
• ISSN: 1365-2672
• Title: Effects of extracellular DNA and DNA-binding protein on the development of a Streptococcus intermedius biofilm
• Volume: 115 1
• Author/Editor: Asikin, Nur; Hirota, Katsuhiko; Yumoto, Hiromichi; Hirao, Kouji; Liu, Dali; Takahashi, Kanako; Murakami, Keiji; Matsuo, Takashi; Shu, Rong; Miyake, Yoichiro
• Author Heading: Asikin, Nur; Hirota, Katsuhiko; Yumoto, Hiromichi; Hirao, Kouji; Liu, Dali; Takahashi, Kanako; Murakami, Keiji; Matsuo, Takashi; Shu, Rong; Miyake, Yoichiro
• Publication, Distribution, etc.: The Society for Applied Microbiology|John Wiley & Sons Inc
• Publication Date: 2013-03-29
• Publication Date (W3CDTF): 2013-03-29
• Alternative Title: Streptococcus intermedius バイオフィルム形成に及ぼす細胞外DNA及びDNA結合タンパク質の影響; Effect of eDNA on biofilm formation
• Periodical title: Journal of Applied Microbiology
• Degree grantor/type: 徳島大学
• Date Granted: 2014-09-10
• Date Granted (W3CDTF): 2014-09-10
• Dissertation Number: 甲第2757号
• Degree Type: 博士（歯学）
• Conferring No. (Dissertation): 甲第2757号
• Text Language Code: eng
• Subject Heading: extracellular DNA; extracellular HLP; biofilm development; Streptococcus intermedius; DNA concentration
• Target Audience: 一般
• Note (General): Aims: The aim of this study was to clarify the effects of homologous and heterologous extracellular DNAs (eDNAs) and histone-like DNA binding protein (HLP) on Streptococcus intermedius biofilm development and rigidity.Methods and Results: Formed biofilm mass was measured with 0.1% crystal violet staining method and observed with a scanning electron microscope. The localizations of eDNA and extracellular HLP (eHLP) in formed biofilm were detected by staining with 7-hydoxyl-9H-(1,3-dichloro-9,9-dimethylacridin-2-one) and anti-HLP antibody without fixation, respectively. DNase I treatment (200 U ml-1) markedly decreased biofilm formation and cell density in biofilms. Co-localization of eHLP and eDNA in biofilm was confirmed. The addition of eDNA (up to 1 μg ml-1) purified from S. intermedius, other Gram-positive, –negative bacteria, or human KB cells into the S. intermedius culture increased the biofilm mass of all tested strains of S. intermedius, wild-type, HLP down-regulated strain, and control strains. In contrast, the addition of eDNA (> 1 μg ml-1) decreased the biofilm mass of all S. intermedius strains. Conclusions: These findings demonstrated that eDNA and eHLP play crucial roles in biofilm development and its rigidity.Significance and Impact of the Study: eDNA- and HLP-targeting strategies may be applicable to novel treatments for bacterial biofilm-related infectious diseases.
• DOI: info:doi/10.1111/jam.12202; https://doi.org/info:doi/10.1111/jam.12202
• Persistent ID (NDL): info:ndljp/pid/11378590; https://dl.ndl.go.jp/pid/11378590
• Collection: 障害者向け資料
• Collection (Materials For Handicapped People:1): テキストデータ
• Collection (particular): 国立国会図書館デジタルコレクション > デジタル化資料 > 博士論文; https://dl.ndl.go.jp/collections/A00014
• Acquisition Basis: 博士論文（自動収集）
• Date Accepted (W3CDTF): 2019-11-04T14:30:53+09:00
• Format (IMT): application/pdf
• Access Restrictions: 国立国会図書館内限定公開
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• Availability of remote photoduplication service: 可
• Periodical Title (URI): http://repo.lib.tokushima-u.ac.jp/106279
• Data Provider (Database): 国立国会図書館 : 国立国会図書館デジタルコレクション; https://dl.ndl.go.jp