脳梗塞発症前運動は、ラットの局所脳虚血後、内在性14-3-3γの増強を介して脳損傷および神経細胞のアポトーシスを減少させる

• Material Type: 博士論文
• Title: 脳梗塞発症前運動は、ラットの局所脳虚血後、内在性14-3-3γの増強を介して脳損傷および神経細胞のアポトーシスを減少させる
• Volume: 224 2
• Author/Editor: 大塚, 章太郎
• Author Heading: 大塚, 章太郎
• Publication, Distribution, etc.: Springer
• Publication Date: 2019; 224 2
• Publication Date (W3CDTF): 2019
• Alternative Title: Preconditioning exercise reduces brain damage and neuronal apoptosis through enhanced endogenous 14-3-3γ after focal brain ischemia in rats
• Periodical title: Brain Structure and Function
• Degree grantor/type: 鹿児島大学
• Date Granted: 2019-03-25
• Date Granted (W3CDTF): 2019-03-25
• Dissertation Number: 甲保研第13号
• Degree Type: 博士（保健学）; Doctor of Philosophy in Health Science
• Conferring No. (Dissertation): 甲保研第13号
• Text Language Code: eng
• Subject Heading: 14-3-3γ; HIF-1α; physical activity; ischemic tolerance; intrinsic factor
• NDC: 498
• Target Audience: 一般
• Note (General): 博士論文全文, 博士論文要旨, 最終試験結果の要旨, 論文審査の要旨; 14-3-3γ is an important early ischemia-inducible protective factor against ischemic cell death in cerebral cortical neurons. We investigated the anti-apoptosis mechanism of enhanced 14-3-3γ mediated by preconditioning exercise-induced brain ischemic tolerance after stroke. Rats were assigned to 4 groups: exercise and ischemia (Ex group), ischemia and no exercise (No-Ex group), exercise and no ischemia (Ex-only group), and no exercise and ischemia (control group). Rats were trained on a treadmill for 5 days a week for 3 weeks (running speed, 25 m/min; running duration, 30 min/day). After the exercise program, stroke was induced by left middle cerebral artery occlusion. The infarct volume, neurological deficits, and motor function, as well as expression levels of hypoxiainduced factor-1α (HIF-1α), 14-3-3γ, P2X7 receptors, p-β-catenin Ser37, Bax, and caspase 3 were evaluated by immunohistochemistry and western blotting. The expression of HIF-1α and 14-3-3γ significantly increased in neurons and astrocytes in the Ex-only group. HIF-1α was co-expressed with P2X7 receptor- and GFAP-positive astrocytes. After stroke, the Ex group had significantly reduced brain infarction. HIF-1α and 14-3-3γ significantly increased in the Ex group compared to the No-Ex group. In addition, p-β-catenin Ser37 significantly increased following elevated 14-3-3γ; in contrast, Bax and caspase 3 were significantly reduced in the Ex group. Our findings suggest that preconditioning exercise prior to ischemia induces neuron- and astrocyte-mediated brain ischemic tolerance through increased expression of HIF-1α and 14-3-3γ, which are intrinsic protective factors; the upregulated 14-3-3γ induced by preconditioning exercise reduces ischemic neuronal cell death through the 14-3-3γ/p-β-catenin Ser37/Bax/caspase 3 anti-apoptotic pathway.
• DOI: info:doi/10.1007/s00429-018-1800-4; https://doi.org/info:doi/10.1007/s00429-018-1800-4
• Persistent ID (NDL): info:ndljp/pid/11426689; https://dl.ndl.go.jp/pid/11426689
• Collection: 障害者向け資料
• Collection (Materials For Handicapped People:1): テキストデータ
• Collection (particular): 国立国会図書館デジタルコレクション > デジタル化資料 > 博士論文; https://dl.ndl.go.jp/collections/A00014
• Acquisition Basis: 博士論文（自動収集）
• Date Accepted (W3CDTF): 2020-01-15T13:48:52+09:00
• Date Created (W3CDTF): 2019-03-22
• Format (IMT): PDF; application/pdf
• Access Restrictions: 国立国会図書館内限定公開
• Service for the Digitized Contents Transmission Service: 図書館・個人送信対象外
• Availability of remote photoduplication service: 可
• Periodical Title (URI): http://hdl.handle.net/10232/00030475
• Data Provider (Database): 国立国会図書館 : 国立国会図書館デジタルコレクション; https://dl.ndl.go.jp

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