Legionella pneumophilaは、マクロファージ様U937細胞に感染するとmiR-218を介してRICTORを制御する 2

• Material Type: 博士論文
• Title: Legionella pneumophilaは、マクロファージ様U937細胞に感染するとmiR-218を介してRICTORを制御する
• Volume: 508 2
• Author/Editor: 郡山, 豊泰
• Author Heading: 郡山, 豊泰
• Publication, Distribution, etc.: Elsevier Inc.
• Publication Date: 2018; 508 2
• Publication Date (W3CDTF): 2018
• Alternative Title: Legionella pneumophila infection-mediated regulation of RICTOR via miR-218 in U937 macrophage cells
• Periodical title: Biochemical and Biophysical Research Communications
• Degree Grantor: 鹿児島大学
• Date Granted: 2019-03-19
• Date Granted (W3CDTF): 2019-03-19
• Dissertation Number: 甲総研第493号
• Degree Type: 博士(医学); Doctor of Philosophy in Medical Science
• Conferring No. (Dissertation): 甲総研第493号
• Text Language Code: eng
• Subject Heading: microRNA; Legionella; Rictor; macrophage
• NDC: 491
• Target Audience: 一般
• Note (General): 博士論文全文, 博士論文要旨, 最終試験結果の要旨, 論文審査の要旨; Background: Inhalation of aerosolized Legionella pneumophila, a Gram-negative bacterium, can cause severe pneumonia. During infection, L. pneumophila replicates intracellularly in macrophages. The involvement of host micro RNAs (miRNAs) in L. pneumophila infection is not fully understood.Methods: The human macrophage-like cell line U937 was infected with L. pneumophila. The levels of miRNA and messenger RNA (mRNAs) were measured using reverse transcriptase polymerase chain reaction. Release of lactate dehydrogenase was used to evaluate cytotoxicity The expression of RICTOR and related proteins was examined by western blotting of cell lysates.Results: L. pneumophila infection upregulated the expression of miR-218 and the host genes SLIT2 and SLIT3 in U937 cells. The expression of RICTOR, a component of the mechanistic target of rapamycin complex 2 (mTORC2), decreased during L. pneumophila infection. RICTOR protein expression was inhibited by the overexpression of miR-218, whereas knockdown of miR-218 restored the downregulation of RICTOR by L. pneumophila . L. pneumophila infection induced the expression of the proinflammatory cytokines IL-6 and TNF alpha, which was modulated by knockdown of miR-218 or RICTOR.Conclusions: Our study revealed the involvement of miR-218 in regulating the inflammatory response of macrophages against L. pneumophila infection. These findings suggest potential novel roles for miR-218 and RICTOR as therapeutic targets of L. pneumophila infection.
• DOI: info:doi/10.1016/j.bbrc.2018.11.093; https://doi.org/info:doi/10.1016/j.bbrc.2018.11.093
• Persistent ID (NDL): info:ndljp/pid/11426691; https://dl.ndl.go.jp/pid/11426691
• Collection: 障害者向け資料
• Collection (Materials For Handicapped People:1): テキストデータ
• Collection (particular): 国立国会図書館デジタルコレクション > デジタル化資料 > 博士論文; https://dl.ndl.go.jp/collections/A00014
• Acquisition Basis: 博士論文（自動収集）
• Date Accepted (W3CDTF): 2020-01-15T13:48:52+09:00
• Date Created (W3CDTF): 2019-04-16
• Format (IMT): PDF; application/pdf
• Access Restrictions: 国立国会図書館内限定公開
• Service for the Digitized Contents Transmission Service: 図書館・個人送信対象外
• Availability of remote photoduplication service: 可
• Original Material (URI): https://www.sciencedirect.com/journal/biochemical-and-biophysical-research-communications
• Periodical Title (URI): http://hdl.handle.net/10232/00030530
• Data Provider (Database): 国立国会図書館 : 国立国会図書館デジタルコレクション; https://dl.ndl.go.jp

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