Cell type diversity in hepatitis B virus RNA splicing and its regulation

• Material Type: 博士論文
• ISSN: 1664-302X
• Title: Cell type diversity in hepatitis B virus RNA splicing and its regulation
• Volume: 10
• Author/Editor: 伊藤, 徳臣
• Author Heading: 伊藤, 徳臣
• Publication, Distribution, etc.: Frontiers Research Foundation
• Publication Date: 2019-02-08
• Publication Date (W3CDTF): 2019-02-08
• Alternative Title: B型肝炎ウイルスRNAスプライシングの細胞型多様性とその制御
• Periodical title: Frontiers in microbiology
• Degree grantor/type: 浜松医科大学
• Date Granted: 2019-03-08
• Date Granted (W3CDTF): 2019-03-08
• Dissertation Number: 甲第794号
• Degree Type: 博士（医学）
• Conferring No. (Dissertation): 甲第794号
• Text Language Code: eng
• Subject Heading: hepatitis B virus; alternative splicing; cis-acting element; intronic silencer; exonic enhancer
• Target Audience: 一般
• Note (General): doctoral; 医学系研究科; Although RNA splicing of hepatitis B virus (HBV) is a commonly observed in livers of hepatitis B patients as well as in the cultured cells replicating the viral genome, its biological significance in the HBV life cycle and the detailed regulatory mechanisms are still largely unclear. In this study, we found cell-type dependency of HBV splicing of the 3.5 kb pregenomic RNA, which is efficiently spliced in human hepatoma cells but not in cells derived from human hepatic stellate, mouse hepatoma and human non-hepatic cells. It may be likely that RNA splicing is one of the determinants of host range restriction of HBV. Given the finding indicating the difference in cell-type dependency of the splicing efficiency between HBV and simian virus 40, we carried out intron-swapping experiments. The results suggest the presence of putative exonic splicing enhancer that possibly works in the cell-type dependent fashion. Together with further mutational analyses, a novel 50-nt intronic splicing silencer, whose secondary structure is well conserved among the HBV strains, was identified. It appears that this intronic silencer functions effectively independent of cell backgrounds.
• DOI: info:doi/10.3389/fmicb.2019.00207; https://doi.org/info:doi/10.3389/fmicb.2019.00207
• Persistent ID (NDL): info:ndljp/pid/11426831; https://dl.ndl.go.jp/pid/11426831
• Collection: 障害者向け資料
• Collection (Materials For Handicapped People:1): テキストデータ
• Collection (particular): 国立国会図書館デジタルコレクション > デジタル化資料 > 博士論文; https://dl.ndl.go.jp/collections/A00014
• Acquisition Basis: 博士論文（自動収集）
• Date Accepted (W3CDTF): 2020-01-15T13:48:52+09:00
• Date Created (W3CDTF): 2019-07-22
• Format (IMT): application/pdf
• Access Restrictions: 国立国会図書館内限定公開
• Service for the Digitized Contents Transmission Service: 図書館・個人送信対象外
• Availability of remote photoduplication service: 可
• Periodical Title (URI): http://hdl.handle.net/10271/00003574; https://hama-med.repo.nii.ac.jp/records/3435
• Data Provider (Database): 国立国会図書館 : 国立国会図書館デジタルコレクション; https://dl.ndl.go.jp

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2023-12-09 再収集