Changes in pulmonary endothelial cell properties during bleomycin-induced pulmonary fibrosis

• Material Type: 博士論文
• ISSN: 1465-9921
• Title: Changes in pulmonary endothelial cell properties during bleomycin-induced pulmonary fibrosis
• Volume: 19 1
• Author/Editor: 加藤, 慎平
• Author Heading: 加藤, 慎平
• Publication, Distribution, etc.: Current Science Ltd.
• Publication Date: 2018-06-26
• Publication Date (W3CDTF): 2018-06-26
• Alternative Title: ブレオマイシン誘導性肺線維症における肺血管内皮細胞の性質の変化
• Periodical title: Respiratory Research
• Degree grantor/type: 浜松医科大学
• Date Granted: 2019-03-13
• Date Granted (W3CDTF): 2019-03-13
• Dissertation Number: 甲第796号
• Degree Type: 博士（医学）
• Conferring No. (Dissertation): 甲第796号
• Text Language Code: eng
• Subject Heading: α-SMA; Bleomycin; Endothelial cell; Fibrosis; Nitric oxide; Prostaglandin I2; TGF-β
• Target Audience: 一般
• Note (General): doctoral; 医学系研究科; Background: Pulmonary fibrosis is a progressive and lethal disease characterized by damage to the lung parenchyma with excess extracellular matrix deposition. The involvement of endothelial cells in fibrosis development is unclear. Methods: We isolated pulmonary endothelial cells, using a magnetic-activated cell sorting system, from mice with pulmonary fibrosis induced by intratracheal bleomycin. We characterized endothelial cells isolated at various times in the course of pulmonary fibrosis development.Results: Inflammatory cell infiltration was observed at 7 days after bleomycin administration, and fibrotic changes with increased collagen content were observed on day 21. Endothelial cells were isolated at these two timepoints. Levels of von Willebrand factor, plasminogen activator inhibitor-1 and matrix metalloproteinase-12 were elevated in lung endothelial cells isolated from bleomycin-treated mice at days 7 and 21. This indicated that intratracheal bleomycin administration induced endothelium injury. Expression of fibrogenic mediators, transforming growth factor (TGF)-β, connective tissue growth factor and platelet-derived growth factor-C was elevated in the cells from bleomycin-treated, compared with untreated, lungs. When endothelial cells were treated with TGF-β, α-smooth muscle actin (SMA) expression and collagen production were increased only in those cells from bleomycin-treated mouse lungs. Thapsigargin-induced prostaglandin I2 and nitric oxide production, decreased in endothelial cells from bleomycin-treated mouse lungs, compared with controls, was further suppressed by TGF-β.Conclusion: Bleomycin administration induced functional changes in lung endothelial cells, indicating potential involvement of endothelium in pulmonary fibrogenesis.
• DOI: info:doi/10.1186/s12931-018-0831-y; https://doi.org/info:doi/10.1186/s12931-018-0831-y
• Persistent ID (NDL): info:ndljp/pid/11429073; https://dl.ndl.go.jp/pid/11429073
• Collection: 障害者向け資料
• Collection (Materials For Handicapped People:1): テキストデータ
• Collection (particular): 国立国会図書館デジタルコレクション > デジタル化資料 > 博士論文; https://dl.ndl.go.jp/collections/A00014
• Acquisition Basis: 博士論文（自動収集）
• Date Accepted (W3CDTF): 2020-01-15T13:48:52+09:00
• Date Created (W3CDTF): 2019-12-05
• Format (IMT): application/pdf
• Access Restrictions: 国立国会図書館内限定公開
• Service for the Digitized Contents Transmission Service: 図書館・個人送信対象外
• Availability of remote photoduplication service: 可
• Periodical Title (URI): http://hdl.handle.net/10271/00003664; https://hama-med.repo.nii.ac.jp/records/3526
• Data Provider (Database): 国立国会図書館 : 国立国会図書館デジタルコレクション; https://dl.ndl.go.jp

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2023-12-09 再収集