Oxidative stress-inducible truncated serine/arginine-rich splicing factor 3 regulates interleukin-8 production in human colon cancer cells 3

• Material Type: 博士論文
• ISSN: 0363-6143
• Title: Oxidative stress-inducible truncated serine/arginine-rich splicing factor 3 regulates interleukin-8 production in human colon cancer cells
• Volume: 306 3
• Author/Editor: Kano, Shizuka; Nishida, Kensei; Kurebe, Hiroyuki; Nishiyama, Chihiro; Kita, Kentaro; Akaike, Yoko; Kajita, Keisuke; Kurokawa, Ken; Masuda, Kiyoshi; Kuwano, Yuki; Tanahashi, Toshihito; Rokutan, Kazuhito
• Author Heading: Kano, Shizuka; Nishida, Kensei; Kurebe, Hiroyuki; Nishiyama, Chihiro; Kita, Kentaro; Akaike, Yoko; Kajita, Keisuke; Kurokawa, Ken; Masuda, Kiyoshi; Kuwano, Yuki; Tanahashi, Toshihito; Rokutan, Kazuhito
• Publication, Distribution, etc.: the American Physiological Society
• Publication Date: 2014-02-01
• Publication Date (W3CDTF): 2014-02-01
• Alternative Title: 酸化ストレスにより誘導されるtruncated serine/arginine –rich splicing factor 3はヒト大腸癌細胞のインターロイキン8の産生を調節する; Truncated SRSF3 regulates IL-8 production
• Periodical title: American Journal of Physiology. Cell Physiology
• Degree grantor/type: 徳島大学
• Date Granted: 2016-03-23
• Date Granted (W3CDTF): 2016-03-23
• Dissertation Number: 甲第2903号
• Degree Type: 博士（医学）
• Conferring No. (Dissertation): 甲第2903号
• Text Language Code: eng
• Subject Heading: SRSF3遺伝子; 酸化ストレス; 選択的スプライシング; ナンセンス変異依存mRNA分解機構; インターロイキン8; SRSF3 gene; oxidative stress; alternative splicing; nonsense-mediated mRNA decay; IL-8
• Target Audience: 一般
• Note (General): Serine/arginine-rich splicing factor 3 (SRSF3) is a member of the SR protein family and plays wide-ranging roles in gene expression. The human SRSF3 gene generates two alternative splice transcripts, a major mRNA isoform (SRSF3-FL) encoding functional full-length protein and a premature termination codon (PTC)-containing isoform (SRSF3-PTC). The latter is degraded through nonsense-mediated mRNA decay (NMD). Treatment of a human colon cancer cell line (HCT116) with 100 μM sodium arsenite increased SRSF3-PTC mRNA levels without changing SRSF3-FL mRNA levels. A chemiluminescence-based NMD reporter assay system demonstrated that arsenite treatment inhibited NMD activity and increased SRSF3-PTC mRNA levels in the cytoplasm, facilitating translation of a truncated SRSF3 protein (SRSF3-TR) from SRSF3-PTC mRNA. SRSF3-TR lacked two-thirds of Arg/Ser-rich (RS) domain whose phosphorylation state is known to be crucial for subcellular distribution. SRSF3-FL was localized in the nucleus, while overexpressed SRSF3-TR was diffusely distributed in the cytoplasm and the nucleus. A part of SRSF3-TR was also associated with stress granules in the cytoplasm. Interestingly, treatment of HCT116 cells with a small interference RNA specifically targeting SRSF3-PTC mRNA significantly attenuated arsenite-stimulated induction of c-JUN protein, its binding activity to the AP-1 binding site (-126 to 120 bp) in the interleukin (IL)-8 gene promoter, and AP-1 promoter activity, resulting in significant reduction of arsenite-stimulated IL-8 production. Our results suggest that SRSF3-TR may function as a positive regulator of oxidative stress-initiated inflammatory responses in colon cancer cells.
• DOI: info:doi/10.1152/ajpcell.00091.2013; https://doi.org/info:doi/10.1152/ajpcell.00091.2013
• Persistent ID (NDL): info:ndljp/pid/11433078; https://dl.ndl.go.jp/pid/11433078
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• Collection (particular): 国立国会図書館デジタルコレクション > デジタル化資料 > 博士論文; https://dl.ndl.go.jp/collections/A00014
• Acquisition Basis: 博士論文（自動収集）
• Date Accepted (W3CDTF): 2020-01-15T13:48:52+09:00
• Format (IMT): application/pdf
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• Periodical Title (URI): http://repo.lib.tokushima-u.ac.jp/109685
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