Characterization of the self-assembly of New Jersey polyomavirus VP1 into virus-like particles and the virus seroprevalence in Japan

• Material Type: 博士論文
• ISSN: 2045-2322
• Title: Characterization of the self-assembly of New Jersey polyomavirus VP1 into virus-like particles and the virus seroprevalence in Japan
• Volume: 9 1
• Author/Editor: 周, 显凤
• Author Heading: 周, 显凤
• Publication, Distribution, etc.: Nature Publishing Group
• Publication Date: 2019-09-11
• Publication Date (W3CDTF): 2019-09-11
• Alternative Title: ニュージャージーポリオーマウイルスVP1のウイルス様粒子への自己集合の解析および日本のおける血清抗体陽性率
• Periodical title: Scientific Reports
• Degree grantor/type: 浜松医科大学
• Date Granted: 2019-10-18
• Date Granted (W3CDTF): 2019-10-18
• Dissertation Number: 乙第566号
• Degree Type: 博士（医学）
• Conferring No. (Dissertation): 乙第566号
• Text Language Code: eng
• Target Audience: 一般
• Note (General): doctoral; 医学系研究科; New Jersey polyomavirus (NJPyV) was discovered in 2014 in a pancreatic transplant recipient’s vascular endothelial cells. Here, in the recombinant baculovirus system, VP1 protein of NJPyV expressed in insect cells was processed. The protein self-assembled into virus-like particles (NJPyV-LPs) in a cell-type-dependent manner, and the particles were then released into the culture media. Spherical ~50-nm-dia. NJPyV-LPs of uniform size with morphology resembling that of the native particles of polyomaviruses were purified from the fraction at 1.33 g/cm3 in supernatants of VP1-expressing Sf9 cells. We investigated the antigenic properties of purified NJPyV-LPs and performed a VLP-based enzyme immunoassay to determine the age-specific prevalence of NJPyV infection in a general Japanese population aged 1–70 years. The overall seropositivity rate of anti-NJPyV antibodies was only 1.8%. This might be explained by the low circulation of NJPyV in Japan. This is the first report of a large-scale serological survey of NJPyV in Asia (n=1,050).
• DOI: info:doi/10.1038/s41598-019-49541-y; https://doi.org/info:doi/10.1038/s41598-019-49541-y
• Persistent ID (NDL): info:ndljp/pid/11458383; https://dl.ndl.go.jp/pid/11458383
• Collection: 障害者向け資料
• Collection (Materials For Handicapped People:1): テキストデータ
• Collection (particular): 国立国会図書館デジタルコレクション > デジタル化資料 > 博士論文; https://dl.ndl.go.jp/collections/A00014
• Acquisition Basis: 博士論文（自動収集）
• Date Accepted (W3CDTF): 2020-03-11T23:38:06+09:00
• Date Created (W3CDTF): 2020-02-26
• Format (IMT): application/pdf
• Access Restrictions: 国立国会図書館内限定公開
• Service for the Digitized Contents Transmission Service: 図書館・個人送信対象外
• Availability of remote photoduplication service: 可
• Periodical Title (URI): http://hdl.handle.net/10271/00003681; https://hama-med.repo.nii.ac.jp/records/3547
• Data Provider (Database): 国立国会図書館 : 国立国会図書館デジタルコレクション; https://dl.ndl.go.jp

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