Three-dimensional periodontal tissue regeneration using a bone-ligament complex cell sheet

• Material Type: 博士論文
• ISSN: 2045-2322
• Title: Three-dimensional periodontal tissue regeneration using a bone-ligament complex cell sheet
• Volume: 10
• Author/Editor: Raju, Resmi; Oshima, Masamitsu; Inoue, Miho; Morita, Tsuyoshi; Huijiao, Yan; Waskitho, Arief; Baba, Otto; Inoue, Masahisa; Matsuka, Yoshizo
• Author Heading: Raju, Resmi; Oshima, Masamitsu; Inoue, Miho; Morita, Tsuyoshi; Huijiao, Yan; Waskitho, Arief; Baba, Otto; Inoue, Masahisa; Matsuka, Yoshizo
• Publication, Distribution, etc.: Springer Nature
• Publication Date: 2020-02-03
• Publication Date (W3CDTF): 2020-02-03
• Alternative Title: 骨－歯根膜線維の複合組織形成による三次元的な歯周組織再生技術の開発
• Periodical title: Scientific Reports
• Degree grantor/type: 徳島大学
• Date Granted: 2020-03-23
• Date Granted (W3CDTF): 2020-03-23
• Dissertation Number: 甲第3381号
• Degree Type: 博士（歯学）
• Conferring No. (Dissertation): 甲第3381号
• Text Language Code: eng
• Subject Heading: Cell sheet; Periodontal tissue; Regeneration; Periodontal ligament cell
• Target Audience: 一般
• Note (General): Periodontal tissue is a distinctive tissue structure composed three-dimensionally of cementum, periodontal ligament (PDL) and alveolar bone. Severe periodontal diseases cause fundamental problems for oral function and general health, and conventional dental treatments are insufficient for healing to healthy periodontal tissue. Cell sheet technology has been used in many tissue regenerations, including periodontal tissue, to transplant appropriate stem/progenitor cells for tissue regeneration of a target site as a uniform tissue. However, it is still difficult to construct a three-dimensional structure of complex tissue composed of multiple types of cells, and the transplantation of a single cell sheet cannot sufficiently regenerate a large-scale tissue injury. Here, we fabricated a three-dimensional complex cell sheet composed of a bone-ligament structure by layering PDL cells and osteoblast-like cells on a temperature responsive culture dish. Following ectopic and orthotopic transplantation, only the complex cell sheet group was demonstrated to anatomically regenerate the bone-ligament structure along with the functional connection of PDL-like fibers to the tooth root and alveolar bone. This study represents successful three-dimensional tissue regeneration of a large-scale tissue injury using a bioengineered tissue designed to simulate the anatomical structure.
• DOI: info:doi/10.1038/s41598-020-58222-0; https://doi.org/info:doi/10.1038/s41598-020-58222-0
• Persistent ID (NDL): info:ndljp/pid/11490182; https://dl.ndl.go.jp/pid/11490182
• Collection: 障害者向け資料
• Collection (Materials For Handicapped People:1): テキストデータ
• Collection (particular): 国立国会図書館デジタルコレクション > デジタル化資料 > 博士論文; https://dl.ndl.go.jp/collections/A00014
• Acquisition Basis: 博士論文（自動収集）
• Date Accepted (W3CDTF): 2020-05-25T09:38:44+09:00
• Format (IMT): application/pdf
• Access Restrictions: 国立国会図書館内限定公開
• Service for the Digitized Contents Transmission Service: 図書館・個人送信対象外
• Availability of remote photoduplication service: 可
• Periodical Title (URI): https://repo.lib.tokushima-u.ac.jp/114213
• Data Provider (Database): 国立国会図書館 : 国立国会図書館デジタルコレクション; https://dl.ndl.go.jp