The protective effect of epigallocatechin 3-gallate on mouse pancreatic islets via the Nrf2 pathway

• Material Type: 博士論文
• ISSN: 0941-1291
• Title: The protective effect of epigallocatechin 3-gallate on mouse pancreatic islets via the Nrf2 pathway
• Volume: 49 6
• Author/Editor: Takata, Atsushi; Wada, Yuma; Ikemoto, Tetsuya; Morine, Yuji; Imura, Satoru; Iwahashi, Shuichi; Saito, Yu; Shimada, Mitsuo
• Author Heading: Takata, Atsushi; Wada, Yuma; Ikemoto, Tetsuya; Morine, Yuji; Imura, Satoru; Iwahashi, Shuichi; Saito, Yu; Shimada, Mitsuo
• Publication, Distribution, etc.: Springer|Japan Surgical Society
• Publication Date: 2019-02-07
• Publication Date (W3CDTF): 2019-02-07
• Alternative Title: Epigallocatechin 3-gallateのNrf2経路を介したマウス膵島細胞保護作用に関する検討; protective effect of EGCG on mouse islets
• Periodical title: Surgery Today
• Degree grantor/type: 徳島大学
• Date Granted: 2019-12-26
• Date Granted (W3CDTF): 2019-12-26
• Dissertation Number: 甲第3342号
• Degree Type: 博士（医学）
• Conferring No. (Dissertation): 甲第3342号
• Text Language Code: eng
• Subject Heading: Epigallocatechin 3-gallate (EGCG); Nrf2; islet transplantation; Reactive oxygen species (ROS); Pre-conditioning; Nuclear factor erythroid 2-related factor 2 (Nrf2); Reactive oxygen species (ROS) production
• Target Audience: 一般
• Note (General): Purpose: Epigallocatechin 3-gallate (EGCG), a green tea polyphenol, has been shown to have anti-oxidant and anti-inflammatory effects in vitro and in vivo. The aim of this study was to investigate the effects and mechanism of EGCG on isolated pancreatic islets as pre-conditioning for pancreatic islet transplantation.Methods: The pancreatic islets were divided into two groups: an islet culture medium group (control) and an islet culture medium with EGCG (100 μM) group. We investigated the islet viability, Nrf2 expression, reactive oxygen species (ROS) production, and heme oxygenase-1 (HO-1) mRNA. Five hundred islet equivalents after 12 h of culture for the EGCG 100 μM and control group were transplanted under the kidney capsule of streptozotocin (STZ)-induced diabetic ICR mice.Results: The cell viability and insulin secretion ability in the EGCG group were preserved, and the nuclear translocation of Nrf2 was increased in the EGCG group (p<0.01). While the HO-1 mRNA levels were also higher in the EGCG group than in the control group (p<0.05), the ROS production was lower (p<0.01). An in vivo functional assessment showed that the blood glucose level had decreased in the EGCG group after transplantation (p<0.01).Conclusion: EGCG protects the viability and function of islets by suppressing ROS production via the Nrf2 pathway.
• DOI: info:doi/10.1007/s00595-019-1761-0; https://doi.org/info:doi/10.1007/s00595-019-1761-0
• Persistent ID (NDL): info:ndljp/pid/11490183; https://dl.ndl.go.jp/pid/11490183
• Collection: 障害者向け資料
• Collection (Materials For Handicapped People:1): テキストデータ
• Collection (particular): 国立国会図書館デジタルコレクション > デジタル化資料 > 博士論文; https://dl.ndl.go.jp/collections/A00014
• Acquisition Basis: 博士論文（自動収集）
• Date Accepted (W3CDTF): 2020-05-25T09:38:44+09:00
• Format (IMT): application/pdf
• Access Restrictions: 国立国会図書館内限定公開
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• Periodical Title (URI): http://repo.lib.tokushima-u.ac.jp/114154
• Data Provider (Database): 国立国会図書館 : 国立国会図書館デジタルコレクション; https://dl.ndl.go.jp