PITHD1 is a proteasome-interacting protein essential for male fertilization

• Material Type: 博士論文
• ISSN: 1083-351X
• Title: PITHD1 is a proteasome-interacting protein essential for male fertilization
• Volume: 295 6
• Author/Editor: Kondo, Hiroyuki; Matsumura, Takafumi; Kaneko, Mari; Inoue, Kenichi; Kosako, Hidetaka; Ikawa, Masahito; Takahama, Yousuke; Ohigashi, Izumi
• Author Heading: Kondo, Hiroyuki; Matsumura, Takafumi; Kaneko, Mari; Inoue, Kenichi; Kosako, Hidetaka; Ikawa, Masahito; Takahama, Yousuke; Ohigashi, Izumi
• Publication, Distribution, etc.: American Society for Biochemistry and Molecular Biology
• Publication Date: 2020-01-08
• Publication Date (W3CDTF): 2020-01-08
• Alternative Title: 精巣内プロテアソームに会合する分子PITHD1の機能解析; PITHD1 is essential for male fertilization
• Periodical title: Journal of Biological Chemistry
• Degree grantor/type: 徳島大学
• Date Granted: 2020-02-27
• Date Granted (W3CDTF): 2020-02-27
• Dissertation Number: 甲第3360号
• Degree Type: 博士（医学）
• Conferring No. (Dissertation): 甲第3360号
• Text Language Code: eng
• Subject Heading: proteasome; thymus; epithelial cell; testis; sperm; fertilization
• Target Audience: 一般
• Note (General): The proteasome is a protein-degrading molecular complex that is necessary for protein homeostasis and various biological functions, including cell cycle regulation, signal transduction, and immune response. Proteasome activity is finely regulated by a variety of proteasome-interacting molecules. PITHD1 is a recently described molecule that has a domain putatively capable of interacting with the proteasome. However, it is unknown as to whether PITHD1 can actually bind to proteasomes and what it does in vivo. Here we report that PITHD1 is detected specifically in the spermatids in the testis and the cortical thymic epithelium in the thymus. Interestingly, PITHD1 associates with immunoproteasomes in the testis, but not with thymoproteasomes in the thymus. Mice deficient in PITHD1 exhibit severe male infertility accompanied with morphological abnormalities and impaired motility of spermatozoa. Furthermore, PITHD1 deficiency reduces proteasome activity in the testis and alters the amount of proteins that are important for fertilization capability by the sperm. However, the PITHD1-deficient mice demonstrate no detectable defects in the thymus, including T cell development. Collectively, our results identify PITHD1 as a proteasome-interacting protein that plays a nonredundant role in the male reproductive system.
• DOI: info:doi/10.1074/jbc.RA119.011144; https://doi.org/info:doi/10.1074/jbc.RA119.011144
• Persistent ID (NDL): info:ndljp/pid/11498391; https://dl.ndl.go.jp/pid/11498391
• Collection: 障害者向け資料
• Collection (Materials For Handicapped People:1): テキストデータ
• Collection (particular): 国立国会図書館デジタルコレクション > デジタル化資料 > 博士論文; https://dl.ndl.go.jp/collections/A00014
• Acquisition Basis: 博士論文（自動収集）
• Date Accepted (W3CDTF): 2020-06-07T00:39:19+09:00
• Format (IMT): application/pdf
• Access Restrictions: 国立国会図書館内限定公開
• Service for the Digitized Contents Transmission Service: 図書館・個人送信対象外
• Availability of remote photoduplication service: 可
• Periodical Title (URI): http://repo.lib.tokushima-u.ac.jp/114611
• Data Provider (Database): 国立国会図書館 : 国立国会図書館デジタルコレクション; https://dl.ndl.go.jp