Type III Secretion Effector VopQ of Vibrio parahaemolyticus Modulates Epithelial Cells’ Central Carbon Metabolism

• Material Type: 博士論文
• ISSN: 2379-5042
• Title: Type III Secretion Effector VopQ of Vibrio parahaemolyticus Modulates Epithelial Cells’ Central Carbon Metabolism
• Volume: 5 2
• Author/Editor: Nguyen, Quoc Anh; Shimohata, Takaaki; Hatayama, Sho; Tentaku, Aya; Kido, Junko; Huong Bui, Thi Mai; Uebanso, Takashi; Mawatari, Kazuaki; Takahashi, Akira
• Author Heading: Nguyen, Quoc Anh; Shimohata, Takaaki; Hatayama, Sho; Tentaku, Aya; Kido, Junko; Huong Bui, Thi Mai; Uebanso, Takashi; Mawatari, Kazuaki; Takahashi, Akira
• Publication, Distribution, etc.: American Society for Microbiology
• Publication Date: 2020-03-18
• Publication Date (W3CDTF): 2020-03-18
• Alternative Title: 腸炎ビブリオ3型分泌装置エフェクタータンパク質VopQは、上皮細胞の糖代謝を変化させる; V. parahaemolyticus effector modulates host metabolism
• Periodical title: mSphere
• Degree grantor/type: 徳島大学
• Date Granted: 2020-03-12
• Date Granted (W3CDTF): 2020-03-12
• Dissertation Number: 甲第3413号
• Degree Type: 博士（栄養学）
• Conferring No. (Dissertation): 甲第3413号
• Text Language Code: eng
• Subject Heading: metabolomics; host-pathogen interaction; Vibrio parahaemolyticus; T3SS effector
• Target Audience: 一般
• Note (General): Vibrio parahaemolyticus is a Gram-negative halophilic pathogen that frequently causing acute gastroenteritis and occasional wound infection. V. parahaemolyticus contains several virulent factors, including Type III secretion systems (T3SSs) and thermostable direct hemolysin (TDH). In particular, T3SS1 is a potent cytotoxic inducer, and T3SS2 is essential for causing acute gastroenteritis. Although much is known about V. parahaemolyticus’s effector manipulating host signaling transductions, little is known about the host metabolomic changes modulated by V. parahaemolyticus. To address this knowledge gap, we performed a metabolomic analysis of the epithelial cells during V. parahaemolyticus infection using capillary electrophoresis–time-of-flight mass spectrometry (CE-TOF/MS). Our results revealed significant metabolomic perturbations upon V. parahaemolyticus infection. Moreover, we identified that T3SS1’s VopQ effector was responsible for inducing the significant metabolic changes in the infected cells. The VopQ effector dramatically altered the host cell’s glycolytic, tricarboxylic acid cycle (TCA), amino acid metabolisms. VopQ effector disrupted host cell redox homeostasis by depleting cellular glutathione and subsequently increasing the level of reactive oxygen species (ROS) production.
• DOI: info:doi/10.1128/mSphere.00960-19; https://doi.org/info:doi/10.1128/mSphere.00960-19
• Persistent ID (NDL): info:ndljp/pid/11498397; https://dl.ndl.go.jp/pid/11498397
• Collection: 障害者向け資料
• Collection (Materials For Handicapped People:1): テキストデータ
• Collection (particular): 国立国会図書館デジタルコレクション > デジタル化資料 > 博士論文; https://dl.ndl.go.jp/collections/A00014
• Acquisition Basis: 博士論文（自動収集）
• Date Accepted (W3CDTF): 2020-06-07T00:39:19+09:00
• Format (IMT): application/pdf
• Access Restrictions: 国立国会図書館内限定公開
• Service for the Digitized Contents Transmission Service: 図書館・個人送信対象外
• Availability of remote photoduplication service: 可
• Periodical Title (URI): http://repo.lib.tokushima-u.ac.jp/114634
• Data Provider (Database): 国立国会図書館 : 国立国会図書館デジタルコレクション; https://dl.ndl.go.jp