Metformin Inhibits Proliferation and Tumor Growth of QGP-1 Pancreatic Neuroendocrine Tumor Cells by Inducing Cell Cycle Arrest and Apoptosis

Persistent ID (NDL): info:ndljp/pid/11511509

Material type: 博士論文

Author: 山名, 浩喜

Publisher: -

Publication date: 2020-03-24

Material Format: Digital

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Name of awarding university/degree: 香川大学,博士（医学）

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Note (General)：: Background/aim: Pancreatic neuroendocrine tumors (pNETs) are rare pancreatic neoplasms, and therapeutic options for pNETs are limited. Metformin is an...

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Material Type: 博士論文
Title: Metformin Inhibits Proliferation and Tumor Growth of QGP-1 Pancreatic Neuroendocrine Tumor Cells by Inducing Cell Cycle Arrest and Apoptosis
Author/Editor: 山名, 浩喜
Author Heading: 山名, 浩喜
Publication Date: 2020-03-24
Publication Date (W3CDTF): 2020-03-24
Degree grantor/type: 香川大学
Date Granted: 2020-03-24
Date Granted (W3CDTF): 2020-03-24
Dissertation Number: 甲第743号
Degree Type: 博士（医学）
Conferring No. (Dissertation): 甲第743号
Text Language Code: eng
Alias of Author: Yamana, Hiroki
Subject Heading: Pancreatic neuroendocrine tumor
apoptosis
cell cycle
metformin
microRNA
Target Audience: 一般
Note (General): Background/aim: Pancreatic neuroendocrine tumors (pNETs) are rare pancreatic neoplasms, and therapeutic options for pNETs are limited. Metformin is an anti-hypoglycemic drug that appears to have anticancer effects. However, little is known about the effect of metformin on pNETs. In this study, we investigated the anti-proliferative effect of metformin on a human pNET cell line.
Materials and methods: The anti-proliferative properties of metformin were evaluated in QGP-1 and NCI-H727 cells using a cell counting kit-8 assay. Xenograft mouse models were used to assess the tumor effect in vivo.
Results: Metformin inhibited the proliferation and anti-tumor growth of QGP-1 cells, accompanied by their arrest during the cell cycle at the G0/G1 phase. Immunohistochemical analysis of tumor tissues revealed down-regulation of cyclin D1 and proliferating cell nuclear antigen in the metformin-treated group. Additionally, metformin induced apoptosis, and the expression of survivin and claspin were decreased in metformin-treated QGP-1 cells according to the apoptosis array. Furthermore, the angiogenic related protein TIMP-1 was down-regulated, and its miRNA expression was altered by metformin in QGP-1 cells.
Conclusion: Taken together, our study demonstrated the therapeutic potential of metformin and provides molecular mechanistic insights into its anti-tumoral effect on pNETs. This study is the first one describing anti-tumoral effects in pNETs.
DOI: info:doi/10.21873/anticanres.13933
https://doi.org/info:doi/10.21873/anticanres.13933
Persistent ID (NDL): info:ndljp/pid/11511509
https://dl.ndl.go.jp/pid/11511509
Collection (particular): 国立国会図書館デジタルコレクション > デジタル化資料 > 博士論文
https://dl.ndl.go.jp/collections/A00014
Acquisition Basis: 博士論文（自動収集）
Date Accepted (W3CDTF): 2020-07-06T20:31:19+09:00
Date Created (W3CDTF): 2020-10-27
Format (IMT): PDF
Access Restrictions: 国立国会図書館内限定公開
Service for the Digitized Contents Transmission Service: 図書館・個人送信対象外
Availability of remote photoduplication service: 可
Periodical Title (URI): https://kagawa-u.repo.nii.ac.jp/records/430
Data Provider (Database): 国立国会図書館 : 国立国会図書館デジタルコレクション
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