Effect of Semaphorin7A during the Effector Phase of Nickel Allergy

• Material Type: 博士論文
• ISSN: 2189-6682
• Title: Effect of Semaphorin7A during the Effector Phase of Nickel Allergy
• Volume: 33 1
• Author/Editor: Minami, Norikazu; Watanabe, Megumi; Liu, Lipei; Yunizar, Mohammad Fadyl; Ichikawa, Tetsuo
• Author Heading: Minami, Norikazu; Watanabe, Megumi; Liu, Lipei; Yunizar, Mohammad Fadyl; Ichikawa, Tetsuo
• Publication, Distribution, etc.: Shikoku Society of Dental Research
• Publication Date: 2020-06-12
• Publication Date (W3CDTF): 2020-06-12
• Alternative Title: ニッケルアレルギー惹起相におけるセマフォリン７Aの効果
• Periodical title: Journal of Oral Health and Biosciences
• Degree grantor/type: 徳島大学
• Date Granted: 2020-05-14
• Date Granted (W3CDTF): 2020-05-14
• Dissertation Number: 甲第3427号
• Degree Type: 博士（歯学）
• Conferring No. (Dissertation): 甲第3427号
• Text Language Code: eng
• Subject Heading: Nickel Allergy; Semaphorin7A; Keratinocyte
• Target Audience: 一般
• Note (General): Background: Metal allergy is caused by many factors, including cells, cytokines, chemokines, or the environment. Recent studies suggested semaphorin7A (Sema7A), expressed on activated T cells, is crucial to produce inflammation through α1β1 integrin on monocytes and macrophages. However, the role of Sema7A on keratinocytes in metal allergy is still unclear. In this study, we focused on keratinocytes since they are known as an important player for skin immunity, and analyzed the effect of Sema7A expressed on keratinocytes in the development of metal allergy.Materials and Methods: Mouse keratinocyte line PAM2.12 cells were treated with NiCl2 to analyze the expression of Sema7A. Ni allergy was induced in female C57BL/6J mice (6-8 weeks old) with or without Sema7A suppression to confirm if Sema7A is necessary to produce allergic reactions to NiCl2.Results: NiCl2 enhanced the expression of Sema7A in a dose and time-dependent manner after 72 hours of stimulation. PAM 2.12 produced TNF-α in response to NiCl2, and this secretion was reduced by Sema7A inhibition. In a mouse model, ear thickness, at 48 hours after NiCl2 injection, was significantly decreased by Sema7A siRNA administration.Conclusions: Sema7A is essential to produce an allergic reaction to NiCl2, especially during the effector phase. Since the interaction between Sema7A and α1β1 integrin enhances inflammation in many skin diseases, this interaction may also have possibilities to be a therapeutic target for metal allergy.
• DOI: info:doi/10.20738/johb.33.1_8; https://doi.org/info:doi/10.20738/johb.33.1_8
• Persistent ID (NDL): info:ndljp/pid/11511524; https://dl.ndl.go.jp/pid/11511524
• Collection: 障害者向け資料
• Collection (Materials For Handicapped People:1): テキストデータ
• Collection (particular): 国立国会図書館デジタルコレクション > デジタル化資料 > 博士論文; https://dl.ndl.go.jp/collections/A00014
• Acquisition Basis: 博士論文（自動収集）
• Date Accepted (W3CDTF): 2020-07-06T20:31:19+09:00
• Format (IMT): application/pdf
• Access Restrictions: 国立国会図書館内限定公開
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• Availability of remote photoduplication service: 可
• Periodical Title (URI): http://repo.lib.tokushima-u.ac.jp/114840
• Data Provider (Database): 国立国会図書館 : 国立国会図書館デジタルコレクション; https://dl.ndl.go.jp