A change in the zinc ion concentration reflects the maturation of insulin-producing cells generated from adipose-derived mesenchymal stem cells

• Material Type: 博士論文
• ISSN: 2045-2322
• Title: A change in the zinc ion concentration reflects the maturation of insulin-producing cells generated from adipose-derived mesenchymal stem cells
• Volume: 9
• Author/Editor: Ohta, Shogo; Ikemoto, Tetsuya; Wada, Yuma; Saito, Yu; Yamada, Shinichiro; Imura, Satoru; Morine, Yuji; Shimada, Mitsuo
• Author Heading: Ohta, Shogo; Ikemoto, Tetsuya; Wada, Yuma; Saito, Yu; Yamada, Shinichiro; Imura, Satoru; Morine, Yuji; Shimada, Mitsuo
• Publication, Distribution, etc.: Springer Nature
• Publication Date: 2019-12-10
• Publication Date (W3CDTF): 2019-12-10
• Alternative Title: 亜鉛イオン濃度変化は脂肪由来幹細胞から作成するインスリン産生細胞の成熟を反映する
• Periodical title: Scientific Reports
• Degree grantor/type: 徳島大学
• Date Granted: 2020-06-25
• Date Granted (W3CDTF): 2020-06-25
• Dissertation Number: 甲第3428号
• Degree Type: 博士（医学）
• Conferring No. (Dissertation): 甲第3428号
• Text Language Code: eng
• Subject Heading: Insulin producing cell (IPC); adipose derived stem cell (ADSC); zinc ion (Zn); maturation; differentiation
• Target Audience: 一般
• Note (General): The generation of insulin-producing cells (IPCs) from pluripotent stem cells could be a breakthrough treatment for type 1 diabetes. However, development of new techniques is needed to exclude immature cells for clinical application. Dithizone staining is used to evaluate IPCs by detecting zinc. We hypothesised that zinc ion (Zn2+) dynamics reflect the IPC maturation status. Human adipose-derived stem cells were differentiated into IPCs by our two-step protocol using two-dimensional (2D) or 3D culture. The stimulation indexes of 2D -and 3D-cultured IPCs on day 21 were 1.21 and 3.64 (P < 0.05), respectively. The 3D-cultured IPCs were stained with dithizone during culture, and its intensity calculated by ImageJ reached the peak on day 17 (P < 0.05). Blood glucose levels of streptozotocin-induced diabetic nude mice were normalised (4/4,100%) after transplantation of 96 3D-cultured IPCs. Zn2+ concentration changes in the medium of 3D cultures had a negative value in the early period and a large positive value in the latter period. This study suggests that Zn2+ dynamics based on our observations and staining of zinc transporters have critical roles in the differentiation of IPCs, and that their measurement might be useful to evaluate IPC maturation as a non-destructive method.
• DOI: info:doi/10.1038/s41598-019-55172-0; https://doi.org/info:doi/10.1038/s41598-019-55172-0
• Persistent ID (NDL): info:ndljp/pid/11526013; https://dl.ndl.go.jp/pid/11526013
• Collection: 障害者向け資料
• Collection (Materials For Handicapped People:1): テキストデータ
• Collection (particular): 国立国会図書館デジタルコレクション > デジタル化資料 > 博士論文; https://dl.ndl.go.jp/collections/A00014
• Acquisition Basis: 博士論文（自動収集）
• Date Accepted (W3CDTF): 2020-08-11T22:41:34+09:00
• Format (IMT): application/pdf
• Access Restrictions: 国立国会図書館内限定公開
• Service for the Digitized Contents Transmission Service: 図書館・個人送信対象外
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• Periodical Title (URI): http://repo.lib.tokushima-u.ac.jp/114940
• Data Provider (Database): 国立国会図書館 : 国立国会図書館デジタルコレクション; https://dl.ndl.go.jp