Proteogenomic analysis of granulocyte macrophage colony- stimulating factor autoantibodies in the blood of a patient with autoimmune pulmonary alveolar proteinosis

The cover of this title could differ from library to library.

Available in National Diet Library

Find on the publisher's website

国立国会図書館デジタルコレクション

Digital data available

Check on the publisher's website

DOI[info:doi/10.1038/s41598-020-61934-y]to the data of the same series

Proteogenomic analysis of granulocyte macrophage colony- stimulating factor autoantibodies in the blood of a patient with autoimmune pulmonary alveolar proteinosis

Persistent ID (NDL): info:ndljp/pid/11538617

Material type: 博士論文

Author: Hashimoto, Atsushi

Publisher: -

Publication date: 2020-03-23

Material Format: Digital

Capacity, size, etc.: -

Name of awarding university/degree: 新潟大学,博士(医学)

View All

Notes on use at the National Diet Library

本資料は、掲載誌(URI)等のリンク先にある学位授与機関のWebサイトやCiNii Dissertationsから、本文を自由に閲覧できる場合があります。

Notes on use

Note (General)：: Recently, attempts to reveal the structures of autoantibodies comprehensively using improved proteogenomics technology, have become popular. This tech...

Search by Bookstore

Read this material in an accessible format.

View materials in accessible formats for people with print disabilities (1Type)

Search by Bookstore

Read in Disability Resources

Other Services
- テキストデータ check it on 国立国会図書館デジタルコレクション

Bibliographic Record

You can check the details of this material, its authority (keywords that refer to materials on the same subject, author's name, etc.), etc.

Digital

Material Type: 博士論文
Title: Proteogenomic analysis of granulocyte macrophage colony- stimulating factor autoantibodies in the blood of a patient with autoimmune pulmonary alveolar proteinosis
Author/Editor: Hashimoto, Atsushi
Publication Date: 2020-03-23
Publication Date (W3CDTF): 2020-03-23
Alternative Title: 肺胞蛋白症患者血清中GM-CSF自己抗体のプロテオゲノミクス解析
Degree grantor/type: 新潟大学
Date Granted: 2020-03-23
Date Granted (W3CDTF): 2020-03-23
Dissertation Number: 甲第4690号
Degree Type: 博士(医学)
Conferring No. (Dissertation): 甲第4690号
Text Language Code: eng
Note (General): Recently, attempts to reveal the structures of autoantibodies comprehensively using improved proteogenomics technology, have become popular. This technology identifies peptides in highly purified antibodies by using an Orbitrap device to compare spectra from liquid chromatography–tandem mass spectrometry against a cDNA database obtained through next generation sequencing. In this study, we first analyzed granulocyte-macrophage colony-stimulating factor (GM-CSF) autoantibodies in a patient with autoimmune pulmonary alveolar proteinosis, using the trapped ion mobility spectrometry coupled with quadrupole time-of-flight (timsTOF). Pro mass spectrometry. The timsTOF Pro identified peptides that partially matched sequences in up to 156 out of 162 cDNA clones. Complementarity-determining region 3 (CDR3) was fully and partially detected in nine and 132clones, respectively. Moreover, we confirmed one unique framework region 4 (FR4) and at least three unique across CDR3 to FR4 peptides via de novo peptide sequencing. This new technology may thus permit the comprehensive identification of autoantibody structure.
Scientific Reports. 2020, 10(1), 4923.
新大院博(医)甲第924号
開始ページ : 1
終了ページ : 45
DOI: info:doi/10.1038/s41598-020-61934-y
https://doi.org/info:doi/10.1038/s41598-020-61934-y
Persistent ID (NDL): info:ndljp/pid/11538617
https://dl.ndl.go.jp/pid/11538617
Collection: 障害者向け資料
Collection (Materials For Handicapped People:1): テキストデータ
Collection (particular): 国立国会図書館デジタルコレクション > デジタル化資料 > 博士論文
https://dl.ndl.go.jp/collections/A00014
Acquisition Basis: 博士論文（自動収集）
Date Accepted (W3CDTF): 2020-09-07T06:04:08+09:00
Date Created (W3CDTF): 2020-08-31
Format (IMT): application/pdf
Access Restrictions: 国立国会図書館内限定公開
Service for the Digitized Contents Transmission Service: 図書館・個人送信対象外
Availability of remote photoduplication service: 可
Periodical Title (URI): http://hdl.handle.net/10191/00051813
Data Provider (Database): 国立国会図書館 : 国立国会図書館デジタルコレクション
https://dl.ndl.go.jp