Phosphorylation sites of microtubuleassociated protein 1B (MAP 1B) are involved in axon growth and regeneration

• Material Type: 博士論文
• Title: Phosphorylation sites of microtubuleassociated protein 1B (MAP 1B) are involved in axon growth and regeneration
• Author/Editor: Ishikawa, Yuya
• Publication Date: 2020-03-23
• Publication Date (W3CDTF): 2020-03-23
• Alternative Title: 軸索伸長・再生に関与する微小管重合促進タンパク質MAP 1Bのリン酸化部位
• Degree grantor/type: 新潟大学
• Date Granted: 2020-03-23
• Date Granted (W3CDTF): 2020-03-23
• Dissertation Number: 甲第4711号
• Degree Type: 博士(医学)
• Conferring No. (Dissertation): 甲第4711号
• Text Language Code: eng
• Subject Heading: Phosphorylation; MAP 1B; Development; Growth cone; Axon regeneration
• Note (General): The growth cone is a specialized structure that forms at the tip of extending axons in developing and regenerating neurons. This structure is essential for accurate synaptogenesis at developmental stages, and is also involved in plasticity-dependent synaptogenesis and axon regeneration in the mature brain. Thus, understanding the molecular mechanisms utilized by growth cones is indispensable to understanding neuronal network formation and rearrangement. Phosphorylation is the most important and commonly utilized protein modification in signal transduction. We previously identified microtubule-associated protein 1B (MAP 1B) as the most frequently phosphorylated protein among ~ 1200 phosphorylated proteins. MAP 1B has more than 10 phosphorylation sites that were present more than 50 times among these 1200 proteins. Here, we produced phospho-specific antibodies against phosphorylated serines at positions 25 and 1201 of MAP 1B that specifically recognize growing axons both in cultured neurons and in vivo in various regions of the embryonic brain. Following sciatic nerve injury, immunoreactivity with each antibody increased compared to the sham operated group. Experiments with transected and sutured nerves revealed that regenerating axons were specifically recognized by these antibodies. These results suggest that these MAP 1B phosphorylation sites are specifically involved in axon growth and that phospho-specific antibodies against MAP 1B are useful markers of growing/regenerating axons.; Molecular Brain. 2019, 12, 93.; 新大院博(医)甲第945号
• DOI: info:doi/10.1186/s13041-019-0510-z; https://doi.org/info:doi/10.1186/s13041-019-0510-z
• Persistent ID (NDL): info:ndljp/pid/11538637; https://dl.ndl.go.jp/pid/11538637
• Collection: 障害者向け資料
• Collection (Materials For Handicapped People:1): テキストデータ
• Collection (particular): 国立国会図書館デジタルコレクション > デジタル化資料 > 博士論文; https://dl.ndl.go.jp/collections/A00014
• Acquisition Basis: 博士論文（自動収集）
• Date Accepted (W3CDTF): 2020-09-07T06:04:08+09:00
• Date Created (W3CDTF): 2020-08-31
• Format (IMT): application/pdf
• Access Restrictions: 国立国会図書館内限定公開
• Service for the Digitized Contents Transmission Service: 図書館・個人送信対象外
• Availability of remote photoduplication service: 可
• Periodical Title (URI): http://hdl.handle.net/10191/00051834
• Data Provider (Database): 国立国会図書館 : 国立国会図書館デジタルコレクション; https://dl.ndl.go.jp