Efficient Prostate Cancer Therapy with Tissue-Specific Homing Peptides Identified by Advanced Phage Display Technology

• Material Type: 博士論文
• Title: Efficient Prostate Cancer Therapy with Tissue-Specific Homing Peptides Identified by Advanced Phage Display Technology
• Volume: 12
• Author/Editor: 和田, 晃典; WADA, Akinori
• Author Heading: 和田, 晃典; WADA, Akinori
• Publication, Distribution, etc.: Cell Press
• Publication Date: 2019-03-29
• Publication Date (W3CDTF): 2019-03-29
• Periodical title: Molecular therapy oncolytics
• Degree grantor/type: 滋賀医科大学
• Date Granted: 2019-09-11
• Date Granted (W3CDTF): 2019-09-11
• Dissertation Number: 甲第845号
• Degree Type: 博士（医学）
• Conferring No. (Dissertation): 甲第845号
• Text Language Code: eng
• Subject Heading: drug delivery system; homing peptide; negative selection; phage display; prostate cancer; targeting.
• Target Audience: 一般
• Note (General): Selective targeting of drugs to tumor cells is a key goal in oncology. Here, we performed an in vivo phage display to identify peptides that specifically target xenografted prostate cancer cells. This yielded three peptide candidates, LN1 (C-TGTPARQ-C), LN2 (C-KNSMFAT-C), and LN3 (C-TNKHSPK-C); each of these peptides was synthesized and evaluated for binding and biological activity. LN1 showed the highest avidity for LNCaP prostate cancer cells in vitro and was thus administered to tumor-bearing mice to evaluate in vivo binding. Strikingly, LN1 specifically bound to the tumor tissue and exhibited very low reactivity with normal liver and kidney tissues. To demonstrate that LN1 could specifically deliver drugs to prostate cancer tissue, a therapeutic peptide, LN1-KLA (C-TGTPARQ-C-GGG-D[KLAKLAK]2), was prepared and used to treat LNCaP cells in vitro and was also administered to tumor-bearing mice. The therapeutic peptide significantly suppressed growth of the cells both in vitro and in vivo. Our study shows that a selective homing peptide strategy could facilitate cell-specific targeting of therapeutics while avoiding adverse reactions in normal tissues.; Thesis or Dissertation; 令和元年度; 10.1016/j.omto.2019.01.001; 博士論文要旨
• DOI: info:doi/10.1016/j.omto.2019.01.001; https://doi.org/info:doi/10.1016/j.omto.2019.01.001
• Persistent ID (NDL): info:ndljp/pid/11551844; https://dl.ndl.go.jp/pid/11551844
• Collection: 障害者向け資料
• Collection (Materials For Handicapped People:1): テキストデータ
• Collection (particular): 国立国会図書館デジタルコレクション > デジタル化資料 > 博士論文; https://dl.ndl.go.jp/collections/A00014
• Acquisition Basis: 博士論文（自動収集）
• Date Accepted (W3CDTF): 2020-10-06T21:18:06+09:00
• Date Created (W3CDTF): 2020-09-17
• Format (IMT): application/pdf
• Access Restrictions: 国立国会図書館内限定公開
• Service for the Digitized Contents Transmission Service: 図書館・個人送信対象外
• Availability of remote photoduplication service: 可
• Related Material (URI): http://hdl.handle.net/10422/00012652
• Periodical Title (URI): http://hdl.handle.net/10422/00012793; https://shiga-med.repo.nii.ac.jp/records/3678
• Data Provider (Database): 国立国会図書館 : 国立国会図書館デジタルコレクション; https://dl.ndl.go.jp

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