Generation of an OCT3/4 reporter cynomolgus monkey ES cell line using CRISPR/Cas9.

• Material Type: 博士論文
• ISSN: 1873-5061
• Title: Generation of an OCT3/4 reporter cynomolgus monkey ES cell line using CRISPR/Cas9.
• Volume: 37
• Author/Editor: 小林, 憲市; KOBAYASHI, Kenichi
• Author Heading: 小林, 憲市; KOBAYASHI, Kenichi
• Publication, Distribution, etc.: Elsevier
• Publication Date: 2019-05
• Publication Date (W3CDTF): 2019-05
• Periodical title: Stem Cell Research
• Degree grantor/type: 滋賀医科大学
• Date Granted: 2019-09-11
• Date Granted (W3CDTF): 2019-09-11
• Dissertation Number: 甲第847号
• Degree Type: 博士（医学）
• Conferring No. (Dissertation): 甲第847号
• Text Language Code: eng
• Target Audience: 一般
• Note (General): Cynomolgus monkey ES (Cyn ES) cells can be generated in a similar manner as human ES cells. However, Cyn ES cells are difficult to maintain in an undifferentiated state by untrained researchers. For easier culture, we generated an OCT3/4-P2A tdTomato IRES ZeocinR Cyn ES cell line using CRISPR/Cas9 genome editing technology. The stop codon of the endogenous OCT3/4 locus was replaced with the P2A tdTomato IRES ZeocinR pA cassette by homologous recombination. This cell line enables us to isolate pluripotent stem cells and exclude differentiated cells by addition of zeocin, especially for culture without feeder cells.; Thesis or Dissertation; 令和元年度; 10.1016/j.scr.2019.101439; 博士論文要旨
• DOI: info:doi/10.1016/j.scr.2019.101439; https://doi.org/info:doi/10.1016/j.scr.2019.101439
• Persistent ID (NDL): info:ndljp/pid/11551846; https://dl.ndl.go.jp/pid/11551846
• Collection: 障害者向け資料
• Collection (Materials For Handicapped People:1): テキストデータ
• Collection (particular): 国立国会図書館デジタルコレクション > デジタル化資料 > 博士論文; https://dl.ndl.go.jp/collections/A00014
• Acquisition Basis: 博士論文（自動収集）
• Date Accepted (W3CDTF): 2020-10-06T21:18:06+09:00
• Date Created (W3CDTF): 2020-09-17
• Format (IMT): application/pdf
• Access Restrictions: 国立国会図書館内限定公開
• Service for the Digitized Contents Transmission Service: 図書館・個人送信対象外
• Availability of remote photoduplication service: 可
• Related Material (URI): http://hdl.handle.net/10422/00012654
• Periodical Title (URI): http://hdl.handle.net/10422/00012795; https://shiga-med.repo.nii.ac.jp/records/3681
• Data Provider (Database): 国立国会図書館 : 国立国会図書館デジタルコレクション; https://dl.ndl.go.jp

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