Host factors influence Barrett's carcinogenesis: findings from a mouse gastroduodenal reflux model.

• Material Type: 博士論文
• ISSN: 1612-9059
• Title: Host factors influence Barrett's carcinogenesis: findings from a mouse gastroduodenal reflux model.
• Volume: 16 3
• Author/Editor: 金井, 俊平; KANAI, Shunpei
• Author Heading: 金井, 俊平; KANAI, Shunpei
• Publication, Distribution, etc.: Springer Tokyo
• Publication Date: 2019-02-21
• Publication Date (W3CDTF): 2019-02-21
• Periodical title: Esophagus
• Degree grantor/type: 滋賀医科大学
• Date Granted: 2019-09-11
• Date Granted (W3CDTF): 2019-09-11
• Dissertation Number: 甲第849号
• Degree Type: 博士（医学）
• Conferring No. (Dissertation): 甲第849号
• Text Language Code: eng
• Subject Heading: Barrett’s esophagus; Bile acids; Carcinogenesis; Mouse; Taurine conjugate
• Target Audience: 一般
• Note (General): Background:Rat gastroduodenal reflux models have been used for analyzing Barrett's carcinogenesis. Mice seem to be more useful than rats for studies targeting genes.; Methods:We induced gastroduodenal contents reflux by esophagojejunostomy using C57BL/6J mice. Mice were divided into a standard diet and high-fat diet groups and kept for 60 weeks. Bile was sampled from the gallbladder to analyze bile acid fractions, and the esophagus was removed for a histological investigation. Human esophagogastric junction adenocarcinoma cells (OE19) were exposed to taurocholic acid (TCA), after which cell proliferative activity was measured. Rat esophageal cancer cell lines, ESCC-DR and ESCC-DRtca with higher malignant potential induced by continuous TCA exposure, were used to perform comprehensive genetic analysis (CGH).; Results:Barrett's epithelium onset occurred in all mice, and no differences in histological changes were noted between the standard diet and high-fat diet groups. However, no development of adenocarcinoma was noted. Most of the mouse bile acid was taurine conjugates. In the experiment using OE-19 cells, TCA promotes cell proliferation in a dose-dependent manner. Array CGH analysis revealed a large number of chromosomal abnormalities in the ESCC-DR, in addition to genetic abnormalities such as in the UGT2B gene, the substrate of which is bile acid. TCA administration resulted in more chromosomal abnormalities being detected.; Conclusions:We showed the effects of TCA in cancer progression in vitro. However, Barrett's adenocarcinoma onset rates differ between mice and rats despite undergoing similar reflux stimulation including taurine-conjugated bile acids being detected in mouse bile juice. These results suggest that host factors seem to influence Barrett's carcinogenesis.; Thesis or Dissertation; 令和元年度; 10.1007/s10388-019-00660-5; 博士論文要旨
• DOI: info:doi/10.1007/s10388-019-00660-5; https://doi.org/info:doi/10.1007/s10388-019-00660-5
• Persistent ID (NDL): info:ndljp/pid/11551847; https://dl.ndl.go.jp/pid/11551847
• Collection: 障害者向け資料
• Collection (Materials For Handicapped People:1): テキストデータ
• Collection (particular): 国立国会図書館デジタルコレクション > デジタル化資料 > 博士論文; https://dl.ndl.go.jp/collections/A00014
• Acquisition Basis: 博士論文（自動収集）
• Date Accepted (W3CDTF): 2020-10-06T21:18:06+09:00
• Date Created (W3CDTF): 2020-09-17
• Format (IMT): application/pdf
• Access Restrictions: 国立国会図書館内限定公開
• Service for the Digitized Contents Transmission Service: 図書館・個人送信対象外
• Availability of remote photoduplication service: 可
• Related Material (URI): http://hdl.handle.net/10422/00012664
• Periodical Title (URI): http://hdl.handle.net/10422/00012796; https://shiga-med.repo.nii.ac.jp/records/3682
• Data Provider (Database): 国立国会図書館 : 国立国会図書館デジタルコレクション; https://dl.ndl.go.jp

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