Alternative Titleモルヒネは血管内皮増殖因子VEGFを抑制することによって、口腔癌細胞HSC-3細胞の活性および細胞増殖を阻害する
Note (General)type:Thesis
Purpose: Although many oral cancer patients require opioids, the effects of morphine and related drugs on oral cancer progression have not been well established. Thus, we examined the effects of morphine exposure on the viability of human oral squamous carcinoma HSC-3 cells and aimed to identify the underlying mechanism. Methods: We exposed HSC-3 cells to the various concentrations of morphine (0, 0.1, 1, 10, 100, or 1000 μmol/L) for 48 h and, subsequently, evaluated cell viability using the 3-[4, 5-dimethylthiazol-2-yl]-2, 5-diphenyl tetrazolium bromide (MTT) assay and cytotoxicity using the lactate dehydrogenase (LDH) assay. To explore the effects of morphine on cell proliferation further, colony formation assay and cell cycle analysis were performed. Additionally, the intracellular expression of nuclear factor kappa B (NF-κB) was analyzed using flow cytometry, and vascular endothelial growth factor (VEGF)-A was evaluated using human VEGF assay. Results: Morphine exposure reduced cell viability and enhanced cytotoxicity in HSC-3 cells in a concentration-dependent manner. The number of colonies in the morphine-treated groups was significantly lower than that in the control group. Consistent with these results, morphine exposure significantly reduced the concentration of VEGF in the cell culture medium in a concentration-dependent manner. However, our data show that morphine at clinical concentrations (0.1-10 μmol/L) does not affect cell cycle and apoptosis. Conclusions: Our results suggest that in human oral cancer HSC-3 cells, morphine exposure inhibits cell viability and growth via suppression of VEGF in clinical conditions.
博士（医学）・甲第727号・令和2年3月16日
© Japanese Society of Anesthesiologists 2019
This is a post-peer-review, pre-copyedit version of an article published in Journal of anesthesia. The final authenticated version is available online at: http://dx.doi.org/10.1007/s00540-019-02645-1.
identifier:Journal of anesthesia Vol.33 No.3 p.408-415 (2019 Jun)
identifier:09138668
identifier:http://ginmu.naramed-u.ac.jp/dspace/handle/10564/3718
identifier:Journal of anesthesia, 33(3): 408-415
DOIinfo:doi/10.1007/s00540-019-02645-1
Collection (particular)国立国会図書館デジタルコレクション > デジタル化資料 > 博士論文
Date Accepted (W3CDTF)2020-10-06T21:18:06+09:00
Data Provider (Database)国立国会図書館 : 国立国会図書館デジタルコレクション