Development of an RT-LAMP assay for the detection of Lassa viruses in southeast and south-central Nigeria

• Material Type: 博士論文
• ISSN: 0166-0934
• Title: Development of an RT-LAMP assay for the detection of Lassa viruses in southeast and south-central Nigeria
• Volume: 269
• Author/Editor: Pemba, Mawonga Christelle
• Author Heading: Pemba, Mawonga Christelle
• Publication, Distribution, etc.: Elsevier B.V.
• Publication Date: 2020-09-18
• Publication Date (W3CDTF): 2020-09-18
• Alternative Title: ナイジェリア南東部および中南部におけるラッサウイルス検出のためのRT-LAMPアッセイの開発
• Periodical title: Journal of Virological Methods
• Degree grantor/type: Nagasaki University (長崎大学)
• Date Granted: 2020-09-18
• Date Granted (W3CDTF): 2020-09-18
• Dissertation Number: 甲医歯薬第1265号
• Degree Type: 博士(医学)
• Conferring No. (Dissertation): 甲医歯薬第1265号
• Text Language Code: eng
• Subject Heading: RT-LAMP; Lassa virus; Nigeria; Molecular diagnosis
• Target Audience: 一般
• Note (General): Lassa virus (LASV) causes Lassa fever (LF), a viral hemorrhagic fever endemic in West Africa. LASV strains are clustered into six lineages according to their geographic location. To confirm a diagnosis of LF, a laboratory test is required. Here, a reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay using a portable device for the detection of LASV in southeast and south-central Nigeria using three primer sets specific for strains clustered in lineage II was developed. The assay detected in vitro transcribed LASV RNAs within 23?min and was further evaluated for detection in 73 plasma collected from suspected LF patients admitted into two health settings in southern Nigeria. The clinical evaluation using the conventional RT-PCR as the reference test revealed a sensitivity of 50% in general with 100% for samples with a viral titer of 9500 genome equivalent copies (geq)/mL and higher. The detection limit was estimated to be 4214 geq/mL. The assay showed 98% specificity with no cross-reactivity to other viruses which cause similar symptoms. These results suggest that this RT-LAMP assay is a useful molecular diagnostic test for LF during the acute phase, contributing to early patient management, while using a convenient device for field deployment and in resource-poor settings.; 長崎大学学位論文 学位記番号:博(医歯薬)甲第1265号 学位授与年月日:令和2年9月18日; Author: Christelle M. Pemba, Yohei Kurosaki, Rokusuke Yoshikawa, Olamide K. Oloniniyi, Shuzo Urata, Maki Sueyoshi, Vahid R. Zadeh, Ifeanyi Nwafor, Michael O. Iroezindu, Nnenna A. Ajayi, Chinedu M. Chukwubike, Nneka M. Chika-Igwenyi, Anne C. Ndu, Damian U. Nwidi, Yuki Maehira, Uche S. Unigwe, Chiedozie K. Ojide, Emeka O. Onwe, Jiro Yasuda; Citation: Journal of Virological Methods, 269, pp.30-37; 2019; identifier:Nagasaki University (長崎大学), 博士(医学) (2020-09-18); http://hdl.handle.net/10069/40269
• DOI: info:doi/10.1016/j.jviromet.2019.04.010; https://doi.org/info:doi/10.1016/j.jviromet.2019.04.010
• Persistent ID (NDL): info:ndljp/pid/11551960; https://dl.ndl.go.jp/pid/11551960
• Collection: 障害者向け資料
• Collection (Materials For Handicapped People:1): テキストデータ
• Collection (particular): 国立国会図書館デジタルコレクション > デジタル化資料 > 博士論文; https://dl.ndl.go.jp/collections/A00014
• Acquisition Basis: 博士論文（自動収集）
• Date Accepted (W3CDTF): 2020-10-06T21:18:06+09:00
• Date Created (W3CDTF): 2020-12-16
• Format (IMT): PDF
• Access Restrictions: 国立国会図書館内限定公開
• Service for the Digitized Contents Transmission Service: 図書館・個人送信対象外
• Availability of remote photoduplication service: 可
• Related Material (URI): http://hdl.handle.net/10069/40269
• Periodical Title (URI): http://hdl.handle.net/10069/40273
• Data Provider (Database): 国立国会図書館 : 国立国会図書館デジタルコレクション; https://dl.ndl.go.jp

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2021-03-07 再収集