Human BST-2/tetherin inhibits Junin virus release from host cells and its inhibition is partially counteracted by viral nucleoprotein 6

• Material Type: 博士論文
• ISSN: 0022-1317
• Title: Human BST-2/tetherin inhibits Junin virus release from host cells and its inhibition is partially counteracted by viral nucleoprotein
• Volume: 101 6
• Author/Editor: Rajabali, Zadeh Vahid
• Author Heading: Rajabali, Zadeh Vahid
• Publication, Distribution, etc.: Microbiology Society
• Publication Date: 2020-09-18
• Publication Date (W3CDTF): 2020-09-18
• Alternative Title: ヒトBST-2/tetherinはフニンウイルスの宿主細胞からの放出を阻害し、その阻害作用はウイルス核タンパク質により部分的に相殺される。
• Periodical title: Journal of General Virology
• Degree grantor/type: Nagasaki University (長崎大学)
• Date Granted: 2020-09-18
• Date Granted (W3CDTF): 2020-09-18
• Dissertation Number: 甲医歯薬第1266号
• Degree Type: 博士(医学)
• Conferring No. (Dissertation): 甲医歯薬第1266号
• Text Language Code: eng
• Subject Heading: Junin virus; Arenaviridae; human BST-2; innate immunity; Tetherin
• Target Audience: 一般
• Note (General): Bone marrow stromal cell antigen-2 (BST-2), also known as tetherin, is an interferon-inducible membrane-associated protein. It effectively targets enveloped viruses at the release step of progeny viruses from host cells, thereby restricting the further spread of viral infection. Junin virus (JUNV) is a member of Arenaviridae, which causes Argentine haemorrhagic fever that is associated with a high rate of mortality. In this study, we examined the effect of human BST-2 on the replication and propagation of JUNV. The production of JUNV Z-mediated virus-like particles (VLPs) was significantly inhibited by over-expression of BST-2. Electron microscopy analysis revealed that BST-2 functions by forming a physical link that directly retains VLPs on the cell surface. Infection using JUNV showed that infectious JUNV production was moderately inhibited by endogenous or exogenous BST-2. We also observed that JUNV infection triggers an intense interferon response, causing an upregulation of BST-2, in infected cells. However, the expression of cell surface BST-2 was reduced upon infection. Furthermore, the expression of JUNV nucleoprotein (NP) partially recovered VLP production from BST-2 restriction, suggesting that the NP functions as an antagonist against antiviral effect of BST-2. We further showed that JUNV NP also rescued the production of Ebola virus VP40-mediated VLP from BST-2 restriction as a broad spectrum BST-2 antagonist. To our knowledge, this is the first report showing that an arenavirus protein counteracts the antiviral function of BST-2.; 長崎大学学位論文 学位記番号:博(医歯薬)甲第1266号 学位授与年月日:令和2年9月18日; Author: Vahid Rajabali Zadeh?, Shuzo Urata?, Miako Sakaguchi, Jiro Yasuda?; Citation: Journal of General Virology, 101(6), pp.573-586; 2020; identifier:Nagasaki University (長崎大学), 博士(医学) (2020-09-18); http://hdl.handle.net/10069/40270
• DOI: info:doi/10.1099/jgv.0.001414; https://doi.org/info:doi/10.1099/jgv.0.001414
• Persistent ID (NDL): info:ndljp/pid/11551961; https://dl.ndl.go.jp/pid/11551961
• Collection: 障害者向け資料
• Collection (Materials For Handicapped People:1): テキストデータ
• Collection (particular): 国立国会図書館デジタルコレクション > デジタル化資料 > 博士論文; https://dl.ndl.go.jp/collections/A00014
• Acquisition Basis: 博士論文（自動収集）
• Date Accepted (W3CDTF): 2020-10-06T21:18:06+09:00
• Date Created (W3CDTF): 2020-12-16
• Format (IMT): PDF
• Access Restrictions: 国立国会図書館内限定公開
• Service for the Digitized Contents Transmission Service: 図書館・個人送信対象外
• Availability of remote photoduplication service: 可
• Related Material (URI): http://hdl.handle.net/10069/40270
• Periodical Title (URI): http://hdl.handle.net/10069/40274
• Data Provider (Database): 国立国会図書館 : 国立国会図書館デジタルコレクション; https://dl.ndl.go.jp

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2021-03-07 再収集