Factors secreted from dental pulp stem cells show multifaceted benefits for treating experimental temporomandibular joint osteoarthritis

• Material Type: 博士論文
• ISSN: 1063-4584
• Title: Factors secreted from dental pulp stem cells show multifaceted benefits for treating experimental temporomandibular joint osteoarthritis
• Volume: 28 6
• Author/Editor: Ogasawara, Naoko; Kano, Fumiya; Hashimoto, Noboru; Mori, Hiroki; Liu, Yao; Xia, L.; Sakamaki, T.; Hibi, H.; Iwamoto, Tsutomu; Tanaka, Eiji; Yamamoto, Akihito
• Author Heading: Ogasawara, Naoko; Kano, Fumiya; Hashimoto, Noboru; Mori, Hiroki; Liu, Yao; Xia, L.; Sakamaki, T.; Hibi, H.; Iwamoto, Tsutomu; Tanaka, Eiji; Yamamoto, Akihito
• Publication, Distribution, etc.: Osteoarthritis Research Society International|Elsevier Ltd.
• Publication Date: 2020-04-06
• Publication Date (W3CDTF): 2020-04-06
• Alternative Title: 歯髄幹細胞からの分泌因子は、顎関節変形性関節症の治療に多面的な効果を示す
• Periodical title: Osteoarthritis and Cartilage
• Degree grantor/type: 徳島大学
• Date Granted: 2020-09-10
• Date Granted (W3CDTF): 2020-09-10
• Dissertation Number: 甲第3444号
• Degree Type: 博士（歯学）
• Conferring No. (Dissertation): 甲第3444号
• Text Language Code: eng
• Subject Heading: Dental pulp stem cell; Conditioned medium; Chondrocyte; Cartilage regeneration; Temporomandibular joint; Osteoarthritis
• Target Audience: 一般
• Note (General): Objective: Temporomandibular joint osteoarthritis (TMJOA) is a degenerative disease characterized by progressive cartilage degeneration, abnormal bone remodeling, and chronic pain. In this study, we aimed to investigate effective therapies to reverse or suppress TMJOA progression.Design: To this end, we performed intravenous administration of serum free conditioned media from human exfoliated deciduous teeth stem cells (SHED-CM) into a mechanical-stress induced murine TMJOA model.Results: SHED-CM administration markedly suppressed temporal muscle inflammation, and improved bone integrity and surface smoothness of the destroyed condylar cartilage. Moreover, SHED-CM treatment decreased the number of IL-1β, iNOS, and MMP-13 expressing chondrocytes, whereas it specifically increased PCNA-positive cells in the multipotent polymorphic cell layer. Notably, the numbers of TdT-mediated dUTP nick end labeling (TUNEL)-positive apoptotic chondrocytes in the SHED-CM treated condyles were significantly lower than in those treated with DMEM, whereas the proteoglycan positive area was restored to a level similar to that of the sham treated group, demonstrating that SHED-CM treatment regenerated the mechanical-stress injured condylar cartilage and subchondral bone. Secretome analysis revealed that SHED-CM contained multiple therapeutic factors that act in osteochondral regeneration.Conclusions: Our data demonstrated that SHED-CM treatment promoted the regeneration and repair of mechanical-stress induced mouse TMJOA. Our observations suggest that SHED-CM has potential to be a potent tissue-regenerating therapeutic agent for patients with severe TMJOA.
• DOI: info:doi/10.1016/j.joca.2020.03.010; https://doi.org/info:doi/10.1016/j.joca.2020.03.010
• Persistent ID (NDL): info:ndljp/pid/11552098; https://dl.ndl.go.jp/pid/11552098
• Collection: 障害者向け資料
• Collection (Materials For Handicapped People:1): テキストデータ
• Collection (particular): 国立国会図書館デジタルコレクション > デジタル化資料 > 博士論文; https://dl.ndl.go.jp/collections/A00014
• Acquisition Basis: 博士論文（自動収集）
• Date Accepted (W3CDTF): 2020-10-06T21:18:06+09:00
• Format (IMT): application/pdf
• Access Restrictions: 国立国会図書館内限定公開
• Service for the Digitized Contents Transmission Service: 図書館・個人送信対象外
• Availability of remote photoduplication service: 可
• Periodical Title (URI): http://repo.lib.tokushima-u.ac.jp/115291
• Data Provider (Database): 国立国会図書館 : 国立国会図書館デジタルコレクション; https://dl.ndl.go.jp