Inorganic polyphosphate potentiates lipopolysaccharideinduced macrophage inflammatory response

• Material Type: 博士論文
• Title: Inorganic polyphosphate potentiates lipopolysaccharideinduced macrophage inflammatory response
• Author/Editor: Ito, Toru
• Publication Date: 2020-09-23
• Publication Date (W3CDTF): 2020-09-23
• Alternative Title: 無機のポリリン酸はリポ多糖がマクロファージに引き起こす炎症反応を増強する
• Degree grantor/type: 新潟大学
• Date Granted: 2020-09-23
• Date Granted (W3CDTF): 2020-09-23
• Dissertation Number: 甲第4798号
• Degree Type: 博士(医学)
• Conferring No. (Dissertation): 甲第4798号
• Text Language Code: eng
• Note (General): Inorganic polyphosphate (polyP) is a linear polymer of orthophosphate units that are linked by phosphoanhydride bonds and is involved in various pathophysiological processes. However, the role of polyP in immune cell dysfunction is not well-understood. In this study, using several biochemical and cell biology approaches, including cytokine assays, immunofluorescence microscopy, receptor-binding assays with quartz crystal microbalance, and dynamic light scanning, we investigated the effect of polyP on in vitro lipopolysaccharide (LPS)- induced macrophage inflammatory response. PolyP up-regulated LPS-induced production of the inflammatory cytokines, such as tumor necrosis factor , interleukin-1, and interleukin-6, in macrophages, and the effect was polyP dose– and chain length– dependent. However, orthophosphate did not exhibit this effect. PolyP enhanced the LPS-induced intracellular macrophage inflammatory signals. Affinity analysis revealed that polyP interacts with LPS, inducing formation of small micelles, and the polyP-LPS complex enhanced the binding affinity of LPS to Toll-like receptor 4 (TLR4) on macrophages. These results suggest that inorganic polyP plays a critical role in promoting inflammatory response by enhancing the interaction between LPS and TLR4 in macrophages.; Journal of Biological Chemistry. 2020, 295(12), 4014-4023.; 新大院博(医)甲第968号; 元資料の権利情報 : 【○!C】 2020 Ito et al. Published under exclusive license by The American Society for Biochemistry and Molecular Biology, Inc. This is an open access article under the CC BY-NC-SA license (https://creativecommons.org/licenses/by-nc-sa/4.0/).
• DOI: info:doi/10.1074/jbc.RA119.011763; https://doi.org/info:doi/10.1074/jbc.RA119.011763
• Persistent ID (NDL): info:ndljp/pid/11583370; https://dl.ndl.go.jp/pid/11583370
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• Collection (particular): 国立国会図書館デジタルコレクション > デジタル化資料 > 博士論文; https://dl.ndl.go.jp/collections/A00014
• Acquisition Basis: 博士論文（自動収集）
• Date Accepted (W3CDTF): 2020-12-08T06:37:43+09:00
• Date Created (W3CDTF): 2020-11-12
• Format (IMT): application/pdf
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• Periodical Title (URI): http://hdl.handle.net/10191/00051959
• Data Provider (Database): 国立国会図書館 : 国立国会図書館デジタルコレクション; https://dl.ndl.go.jp