Proteomic profiling of HTLV-1 carriers and ATL patients reveals sTNFR2 as a novel diagnostic biomarker for acute ATL

• Material Type: 博士論文
• Title: Proteomic profiling of HTLV-1 carriers and ATL patients reveals sTNFR2 as a novel diagnostic biomarker for acute ATL
• Volume: 4 6
• Author/Editor: Guerrero, Carmina Louise Hugo
• Author Heading: Guerrero, Carmina Louise Hugo
• Publication, Distribution, etc.: University of the Ryukyus
• Publication Date: 2020-03-20
• Publication Date (W3CDTF): 2020-03-20
• Periodical title: Blood Advances
• Degree grantor/type: 琉球大学
• Date Granted: 2020-09-18
• Date Granted (W3CDTF): 2020-09-18
• Dissertation Number: 甲第23号
• Degree Type: 博士（保健学）
• Conferring No. (Dissertation): 保健研第23号
• Text Language Code: eng
• Target Audience: 一般
• Note (General): AdultT-cell leukemia/lymphoma (ATL) is ahuman T-cell leukemia virus type 1 (HTLV-1)–associated T-cell malignancy with generally poor prognosis. Although only ;5% of HTLV-1 carriers progress to ATL, early diagnosis is challenging because of the lack of ATL biomarkers. In this study, we analyzed blood plasma profiles of asymptomatic HTLV-1 carriers (ACs); untreated ATL patients, including acute, lymphoma, smoldering, and chronic types; and ATL patients in remission. Through SOMAscan, expression levels of 1305 plasma proteins were analyzed in 85 samples (AC, n 5 40; ATL, n 5 40; remission, n 5 5). Using gene set enrichment analysis and gene ontology, overrepresented pathways in ATL vs AC included angiogenesis, inflammation by cytokines and chemokines, interleukin-6 (IL-6)/JAK/STAT3, and notch signaling. In selecting candidate biomarkers, we focused on soluble tumor necrosis factor receptor 2 (sTNFR2) because of its active role in enriched pathways, extreme significance (Welch’s t test P , .00001), high discrimination capacity (area under the curve .0.90), and novelty in ATL research. Quantification of sTNFR2 in 102 plasma samples (AC, n530; ATL, n568; remission, n54) using enzyme-linked immunosorbent assay showed remarkable elevations in acute ATL, at least 10 times those of AC samples, and return of sTNFR2 to AC state levels after achieving remission. Flow cytometry and immunostaining validated the expression of TNFR2 in ATL cells. No correlation between sIL-2 and sTNFR2 levels in acute ATL was found, suggesting the possibility of sTNFR2 as an independent biomarker. Our findings represent the first extensive blood-based proteomic analysis of ATL, suggesting the potential clinical utility of sTNFR2 in diagnosing acute ATL.; 学位論文
• DOI: info:doi/10.1182/bloodadvances.2019001429; https://doi.org/info:doi/10.1182/bloodadvances.2019001429
• Persistent ID (NDL): info:ndljp/pid/11630050; https://dl.ndl.go.jp/pid/11630050
• Collection: 障害者向け資料
• Collection (Materials For Handicapped People:1): テキストデータ
• Collection (particular): 国立国会図書館デジタルコレクション > デジタル化資料 > 博士論文; https://dl.ndl.go.jp/collections/A00014
• Acquisition Basis: 博士論文（自動収集）
• Date Accepted (W3CDTF): 2021-01-30T08:56:55+09:00
• Format (IMT): application/pdf
• Access Restrictions: 国立国会図書館内限定公開
• Service for the Digitized Contents Transmission Service: 図書館・個人送信対象外
• Availability of remote photoduplication service: 可
• Periodical Title (URI): http://hdl.handle.net/20.500.12000/47433; https://u-ryukyu.repo.nii.ac.jp/records/2011282
• Data Provider (Database): 国立国会図書館 : 国立国会図書館デジタルコレクション; https://dl.ndl.go.jp

Table of Contents

2023-03-01 再収集

2023-03-01 再収集

2023-03-01 再収集

2023-03-01 再収集

2023-09-05 再収集