Association between microRNA‑527 and glypican‑3 in hepatocellular carcinoma

• Material Type: 博士論文
• Title: Association between microRNA‑527 and glypican‑3 in hepatocellular carcinoma
• Author/Editor: 野村, 圭
• Author Heading: 野村, 圭
• Publication Date: 2020-09-16
• Publication Date (W3CDTF): 2020-09-16
• Alternative Title: 肝細胞癌におけるmicroRNA-527とグリピカン-3との関連性
• Degree grantor/type: 香川大学
• Date Granted: 2020-09-16
• Date Granted (W3CDTF): 2020-09-16
• Dissertation Number: 乙第292号
• Degree Type: 博士（医学）
• Conferring No. (Dissertation): 乙第292号
• Text Language Code: eng
• Alias of Author: Nomura, Kei
• Subject Heading: hepatocellular carcinoma; microRNA; glypican 3; microRNA‑527; target gene
• Target Audience: 一般
• Note (General): The present study aimed to identify the specific microRNAs (miRNAs/miRs) and their corresponding target genes involved in hepatocellular carcinomas (HCCs). Microarray analysis was performed to examine the miRNA expression profiles of four paired HCC and corresponding non‑cancerous (N) liver tissues using 985 miRNA probes. The Human miRNA Target database was used to identify the target genes of differentially expressed miRNAs between the HCC and N tissues. The protein expression levels of target genes in the HCC tissues and cell lines were evaluated using western blotting. miRNA‑mediated suppression of target gene expression was evaluated by transiently transfecting the miRNA into the HCC cell lines. Of the 985 miRNAs evaluated, four miRNAs were differentially expressed (three upregulated and one downregulated miRNAs). Of these four miRNAs, miRNA‑527 was highly downregulated in the HCC tissues. Glypican‑3 (GPC‑3) was predicted as a target gene of miRNA‑527. Western blotting revealed that GPC‑3 protein is highly expressed in the HCC tissues and HCC cell lines compared with N and normal cell lines. Transfection with miR‑527 resulted in suppression of GPC‑3 protein expression in the Cos7 cells. Furthermore, transfection with miR‑527 also inhibited the intrinsic expression of GPC‑3 in the Huh‑7 cell line. This indicated that miR‑527 in the HCC tissues may be an important novel miRNA that targets the GPC‑3 gene expression. GPC‑3, whose expression is regulated by miR‑527, may be involved in the development and progression of HCC.; This is the Publisher's Version of the following article: Association between microRNA-527 and glypican-3 in hepatocellular carcinoma; Oncology letters.; Volume 21 Number 3 (2021) doi: 10.3892/ol.2021.12490 , which has been published in final form at https://doi.org/10.3892/ol.2021.12490 .
• DOI: info:doi/10.3892/ol.2021.12490; https://doi.org/info:doi/10.3892/ol.2021.12490
• Persistent ID (NDL): info:ndljp/pid/11659349; https://dl.ndl.go.jp/pid/11659349
• Collection: 障害者向け資料
• Collection (Materials For Handicapped People:1): テキストデータ
• Collection (particular): 国立国会図書館デジタルコレクション > デジタル化資料 > 博士論文; https://dl.ndl.go.jp/collections/A00014
• Acquisition Basis: 博士論文（自動収集）
• Date Accepted (W3CDTF): 2021-04-07T03:01:57+09:00
• Date Created (W3CDTF): 2020-10-20
• Format (IMT): PDF
• Access Restrictions: 国立国会図書館内限定公開
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• Related Material: This is the Publisher's Version of the following article: Association between microRNA-527 and glypican-3 in hepatocellular carcinoma; Oncology letters.; Volume 21 Number 3 (2021) doi: 10.3892/ol.2021.12490 , which has been published in final form at https://doi.org/10.3892/ol.2021.12490 .
• Periodical Title (URI): https://kagawa-u.repo.nii.ac.jp/records/158
• Data Provider (Database): 国立国会図書館 : 国立国会図書館デジタルコレクション; https://dl.ndl.go.jp