Osteoadherin serves roles in the regulation of apoptosis and growth in MC3T3-E1 osteoblast cells

• Material Type: 博士論文
• Title: Osteoadherin serves roles in the regulation of apoptosis and growth in MC3T3-E1 osteoblast cells
• Author/Editor: 浜谷, 絵里
• Author Heading: 浜谷, 絵里
• Publication, Distribution, etc.: Hokkaido University
• Publication Date: 2020-03-25
• Publication Date (W3CDTF): 2020-03-25
• Alternative Title: オステオアドへリンは MC3T3-E1 骨芽細胞におい てアポトーシスと細胞増殖を制御する
• Contributor: 田村, 正人; 網塚, 憲生; 飯村, 忠浩
• Degree grantor/type: 北海道大学
• Date Granted: 2020-03-25
• Date Granted (W3CDTF): 2020-03-25
• Dissertation Number: 甲第13878号
• Degree Type: 博士(歯学)
• Conferring No. (Dissertation): 甲第13878号
• Text Language Code: eng
• NDC: 497
• Target Audience: 一般
• Note (General): Small leucine-rich proteoglycans (SLRPs) are a class of proteoglycans that are characterized by small protein cores and structures of leucine-rich repeats. SLRPs are expressed in most extracellular matrices and share numerous biological functions that are associated with binding of collagens and cell surface receptors. Osteoadherin (also termed osteomodulin) is encoded by the Omd gene and is a keratan sulfate proteoglycan of the class II subfamily of SLRPs. Osteoadherin is highly expressed in mineralized tissues, including bone and dentin; however, it's precise roles remain unknown. The present study determined the Omd expression levels and investigated the effects of over- and under-expression of osteoadherin in osteoblastic cells. Omd mRNA expression increased with osteoblast differentiation in MC3T3-E1 cells. In C2C12 cells, Omd mRNA expression was induced by bone morphogenetic protein (BMP)2. Reporter assays similarly demonstrated activation of the Omd gene promoter following co-transfection with Smad1 and Smad4, which are intracellular signaling molecules of the BMP2 signaling pathway. Overexpression of Omd increased the viability and decreased caspase 3/7 activity in MC3T3-E1 cells. By contrast, following transfection with small interfering RNA for Omd, viable cell numbers were decreased and caspase 3/7 activity was increased. Furthermore, overexpression of Omd reduced the expression of CCN family 2 in these cells. These results demonstrate that Omd expression is regulated during osteoblast differentiation, and that the protein product osteoadherin serves roles in the apoptosis and growth of osteoblast cells.; (主査) 教授 田村 正人, 教授 網塚 憲生, 教授 飯村 忠浩; 歯学研究科（口腔医学専攻）
• DOI: 10.14943/doctoral.k13878; https://doi.org/10.14943/doctoral.k13878
• Persistent ID (NDL): info:ndljp/pid/11660431; https://dl.ndl.go.jp/pid/11660431
• Collection: 障害者向け資料
• Collection (Materials For Handicapped People:1): テキストデータ
• Collection (particular): 国立国会図書館デジタルコレクション > デジタル化資料 > 博士論文; https://dl.ndl.go.jp/collections/A00014
• Acquisition Basis: 博士論文（自動収集）
• Date Accepted (W3CDTF): 2021-04-07T03:01:57+09:00
• Date Created (W3CDTF): 2020-03
• Format (IMT): PDF
• Access Restrictions: 国立国会図書館内限定公開
• Service for the Digitized Contents Transmission Service: 図書館・個人送信対象外
• Availability of remote photoduplication service: 可
• Periodical Title (URI): http://dx.doi.org/10.14943/doctoral.k13878; http://hdl.handle.net/2115/80510
• Is Referenced By (URI): http://hdl.handle.net/2115/78223
• Data Provider (Database): 国立国会図書館 : 国立国会図書館デジタルコレクション; https://dl.ndl.go.jp

• Summary, etc.: Small leucine-rich proteoglycans (SLRPs) are a class of proteoglycans that are characterized by small protein cores and structures of leucine-rich rep...