ナイシンA作用により分離したナイシンA高度耐性MRSAのビルレンスに及ぼす影響

• Material Type: 博士論文
• Title: ナイシンA作用により分離したナイシンA高度耐性MRSAのビルレンスに及ぼす影響
• Author/Editor: 渡邉, 温子
• Author Heading: 渡邉, 温子
• Publication, Distribution, etc.: American Society for Microbiology
• Publication Date: 2020-04-20
• Publication Date (W3CDTF): 2020-04-20
• Alternative Title: An alternative nisin A resistance mechanism affects virulence in Staphylococcus aureus
• Degree grantor/type: 鹿児島大学
• Date Granted: 2020-04-20
• Date Granted (W3CDTF): 2020-04-20
• Dissertation Number: 甲総研第561号
• Degree Type: 博士(歯学); Doctor of Philosophy in Dental Science
• Conferring No. (Dissertation): 甲総研第561号
• Text Language Code: eng
• NDC: 490
• Target Audience: 一般
• Note (General): 博士論文全文, 博士論文要旨, 最終試験結果の要旨, 論文審査の要旨; Nisin A is a bacteriocin produced by Lactococcus lactis and is widely used as a food preservative. Staphylococcus aureus has the BraRS-VraDE system that provides resistance against low concentrations of nisin A. BraRS is a two-component system that induces the expression of the ABC transporter VraDE. Previously, we isolated a highly nisin A-resistant strain with increased VraDE expression due to a mutation in braRS. In this study, we isolated S. aureus MW2 mutants with BraRS-VraDE-independent nisin A resistance. These mutants, designated SAN2 (S. aureus nisin resistant) and SAN469, had a mutation in pmtR, which encodes a transcriptional regulator responsible for the expression of the pmtABCD operon. As a result, these mutants exhibited increased expression of PmtABCD, a transporter responsible for the export of phenol-soluble modulin (PSM). Characterization of the mutants revealed that they have decreased susceptibility to human β-defensin-3 (hBD3) and LL37, which are innate immune factors. Additionally, these mutants showed higher hemolytic activity than the original MW2 strain. Furthermore, in a mouse bacteremia model, the SAN2 strain exhibited a lower survival rate than the original MW2 strain. These results indicate that the increased expression of pmtABCD due to a pmtR mutation is an alternative nisin A resistance mechanism that also affects virulence in S. aureus.Miki Kawada-Matsuo, Atsuko Watanabe, Kaoru Arii, Yuichi Oogai, Kazuyuki Noguchi, Shouichi Miyawaki, Tetsuya Hayashi, Hitoshi KomatsuzawaStaphylococcus aureus Virulence Affected by an Alternative Nisin A Resistance MechanismApplied and Environmental Microbiology 2020DOI: 10.1128/AEM.02923-19
• Persistent ID (NDL): info:ndljp/pid/11671396; https://dl.ndl.go.jp/pid/11671396
• Collection: 障害者向け資料
• Collection (Materials For Handicapped People:1): テキストデータ
• Collection (particular): 国立国会図書館デジタルコレクション > デジタル化資料 > 博士論文; https://dl.ndl.go.jp/collections/A00014
• Acquisition Basis: 博士論文（自動収集）
• Date Accepted (W3CDTF): 2021-05-16T23:51:55+09:00
• Date Created (W3CDTF): 2020-07-08
• Format (IMT): PDF; application/pdf
• Access Restrictions: 国立国会図書館内限定公開
• Service for the Digitized Contents Transmission Service: 図書館・個人送信対象外
• Availability of remote photoduplication service: 可
• Periodical Title (URI): http://hdl.handle.net/10232/00031133
• Data Provider (Database): 国立国会図書館 : 国立国会図書館デジタルコレクション; https://dl.ndl.go.jp

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